Umuwa Home
  Web     Image     Video     News  
 
Showing results for single stranded dna purification

    Web Results
 
 
ssDNA/RNA Clean & Concentrator™ - DNA/RNA Co-Purification ... 
 
www.zymoresearch.com/rna/total-rna-purification/dna-rna...
The ssDNA/RNA Clean & Concentrator™ provides a simple and reliable method for the rapid separation, clean-up, and concentration of up to ~5 µg (per prep.) of single stranded DNA and/or RNA from double stranded species (e.g., genomic DNA). This  
 
 
 
Streamlined method for purifying single-stranded DNA from PCR ... 
 
www.biotechniques.com/BiotechniquesJournal/2005/May/...
Single-stranded DNA (ssDNA) is very useful in diagnosis, molecular therapy, and basic research in molecular biology. When high-throughput or frequent preparation of ssDNA is needed, it is highly desirable to accomplish the task by a procedure that is rapid, efficient, cost-effective, and automation  
 
 
 
High-yield purification of small, single-stranded DNA 
 
www.sciencedirect.com/science/article/pii/0003269781905467
Several simple methods that aid in the high-yield purification of small, single-stranded DNA are described. The methods rely initially on the silicone coating o  
 
 
 
Preparing Single-Stranded DNA Templates | Life Technologies 
 
www.lifetechnologies.com/us/en/home/life-science/dna-rna...
Many applications require the use of single-stranded DNA templates. The immobilization of a double-stranded PCR product for preparation of two single-stranded DNA fragments is probably the most widely used protocol for Dynabeads® M-280 Streptavidin.  
 
 
 
DNA /RNA Co- Purification - DNA Purification 
 
www.zymoresearch.com/dna/dna-rna-co-purification
Zymo Research provides the highest quality Epigenetics, DNA and RNA purification products while ensuring they are both simple and reliable.  
 
 
 
Preparation of single-stranded DNA from PCR products with ... 
 
www.ncbi.nlm.nih.gov/pubmed/22047177
Preparation of single-stranded DNA from PCR products with streptavidin magnetic beads. Wilson R. ... DNA, Single-Stranded/isolation & purification* Immunomagnetic Separation/methods* Polymerase Chain Reaction; Sodium Hydroxide/chemistry*  
 
 
 
Identification and purification single-stranded-DNA-specific ... 
 
www.pnas.org/content/86/8/2627.full.pdf
Purification ofthe single-stranded-DNA-specific nuclease from arecJ-overproducing strain Protein, Activity, Specific activity, Yield, Fraction mg units units/mg I. Crudeextract 50 420,000 8,400 100 II. Ammoniumsulfate 37 470,000 13,000 110 III. PBE94 8.1 310,000 38,000 74  
 
 
 
Preparation of Single-Stranded Bacteriophage M13 DNA by ... 
 
www.molecularcloning.com/index.php?prt=8
Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol (Protocol summary only for purposes of this preview site)  
 
 
 
DNA and RNA Purification: GenElute™ PCR Clean-Up Kit ... 
 
www.sigmaaldrich.com/life-science/molecular-biology/dna...
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts.  
 
 
 
Preparing Single-Stranded DNA Templates - Invitrogen Part of ... 
 
www.b2b.invitrogen.com/site/us/en/home/Products-and...
Many applications require the use of single-stranded DNA templates. The immobilisation of a double-stranded PCR product for preparation of two single-stranded DNA fragments is probably the most widely used protocol for Dynabeads® M-280 Streptavidin.  
 
 
 
Purification of single stranded DNA from asymmetric PCR ... 
 
link.springer.com/article/10.1023/A%3A1008829931836
A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric  
 
 
 
Purification of Single-Stranded M13 DNA by Cooperative Triple ... 
 
www.sciencedirect.com/science/article/pii/S0003269796900536
A solid-phase triple-helix-mediated affinity capture method is described for the purification of single-stranded M13 DNA for use as template in fluorescence-bas  
 
 
 
PCR clean-up Gel extraction - Clontech Laboratories, Inc ... 
 
clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17487
Single stranded DNA clean-up 1 PCR clean-up, DNA clean-up, or single stranded DNA clean-up: Adjust binding condition Gel extraction: ... purification of DNA from samples without SDS the standard protocol for PCR clean-up can be used (see section 5.1).  
 
 
 
Plasmid DNA purification - California Lutheran University 
 
public.callutheran.edu/~revie/protocols/Q-Minipreps.pdf
Purification of plasmid DNA Plasmids are small double-stranded DNA pieces of DNA that exist outside the chromosome of the cell in which they are located.  
 
 
 
single stranded DNA purification using streptavidin agarose ... 
 
molecularstation.com/forum/dna-techniques/66216-single...
Hi all, Did anyone do single stranded dna purification using streptavidin agarose beads before? I PCR amplified a single stranded dna library to get double stranded dna using reverse-biotinylated  
 
 
 
M13 bacteriophage - Wikipedia, the free encyclopedia 
 
en.wikipedia.org/wiki/M13_bacteriophage
M13 is a filamentous bacteriophage composed of circular single stranded DNA which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.  
 
 
 
DNA EXTRACTION METHODS 
 
www.sjsu.edu/.../courses/c3/s0/DNA%20Extraction.ppt
Chelex Extraction The Chelex extraction process denatures double stranded DNA and yields single stranded DNA, ... Different DNA extraction methods used in forensic DNA PowerPoint Presentation What are the essential components of a DNA extraction Procedure?  
 
 
 
purification of long single stranded DNA - Molecular Cloning 
 
www.protocol-online.org/biology-forums/posts/11029.html
Hi, I am not a biologist or biochemist and wonder if I cold get help with the following from an expert: I am trying to purify long (around 1 micrometer say) single stranded DNA, the aim is to get rid of shorter polimerization products primers and nucleotides.  
 
 
 
Reversed-phase ion-pair liquid chromatography method for ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3814375
Single-stranded C18 cartridge DNA purification. The single-stranded oligonucleotides obtained from ~1 µmol syntheses were resuspended in TE buffer [10 mM Tris-HCl, 1 mM ethylenediamine tetraacetic acid (EDTA), pH 8.0] with 150 mM NaCl and purified using Waters C18 Sep-Pak cartridges.  
 
 
 
Deoxyribonuclease I (DNase I) - Pierce Protein Biology ... 
 
www.piercenet.com/product/deoxyribonuclease-i-dnase-i
Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA.  
 
 
 
Single-stranded RNA Synthesis | GE Dharmacon 
 
dharmacon.gelifesciences.com/.../single-strand-rna-synthesis...
Single-stranded RNA can be shipped in its 2' protected form to improve stability. As a result of chemical RNA synthesis, all oligonucleotides will have chemical protecting groups at the 2' position of each nucleotide.  
 
 
 
NucleoSpin Gel and PCR Clean-up - MACHEREY-NAGEL Homepage 
 
www.mn-net.com/tabid/1452/default.aspx
• Separate buffers for single stranded DNA or SDS containing samples available ... • Clean-up of single stranded DNA and RNA extraction from agarose gels ... • NucleoSpin ® Gel and PCR Clean-up ensures complete removal of contaminations such as:  
 
 
 
Can I use spin-column to purify single-stranded DNA? 
 
www.researchgate.net/post/Can_I_use_spin-column_to...
I have a sample of single-stranded cDNA ligated with 3' DNA adapter. Generally, I use phenol/chloroform purification and ethanol precipitation.  
 
 
 
Single-Stranded DNA Isolation & Mutagenesis - DNA Extraction ... 
 
www.biolabprotocols.com/details/11886/Single-Stranded-DNA......
Single-Stranded DNA Isolation & Mutagenesis: ... Receive all current lab protocols on inbox free by entering your email address:  
 
 
 
ReadyAmp™ Genomic DNA Purification System - www.promega.com 
 
www.promega.com/.../readyamp-genomic-dna-purification-system...
System for purification of single-stranded DNA from whole blood or blood stains. DNA may be used directly in amplification reactions without further manipulation. No organic extraction or ethanol precipitation.  
 
 
 
Post Reaction Cleanup in DNA & RNA Purification | Sigma-Aldrich 
 
www.sigmaaldrich.com/.../dna-and-rna-purification/...
Post Reaction Cleanup in DNA & RNA Purification: Sigma-Aldrich provides GenElute™ PCR clean-up, agarose spin columns and minus EtBr spin columns for purification of single or double stranded PCR amplification fragments; as well as extraction of DNA fragments from Ethidium Bromide (EtBr ...  
 
 
 
Single-stranded DNA library preparation for the sequencing of ... 
 
www.nature.com/nprot/journal/v8/n4/full/nprot.2013.038.html
Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA. Marie-Theres Gansauge 1, Matthias Meyer 1, Affiliations; Contributions  
 
 
 
Antibodies to double-stranded DNA: purification and ... 
 
www.jimmunol.org/content/125/2/874
Antibodies to double-stranded DNA: purification and characterization of binding specificities. A C Gilliam, ... that binds only to entirely double-stranded DNA and other types that bind to both double- and single-stranded DNA. We report also that ...    
 
 
QIAquick PCR Purification Kit - QIAGEN 
 
www.qiagen.com/.../dna-cleanup/qiaquick-pcr-purification-kit...
The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.  
 
 
 
Single Stranded Templates for Pyrosequencing | Life Technologies 
 
www.lifetechnologies.com/us/en/home/life-science/dna-rna...
Pyrosequencing™ requires a single stranded sequencing template. The simplest way to prepare this is by the use of Dynabeads M-280 Streptavidin . First the DNA fragment of interest is PCR amplified with one of the primers biotinylated.  
 
 
 
III. Methods for DNA isolation - The University of Oklahoma's ... 
 
www.genome.ou.edu/protocol_book/protocol_partIII.html
III. Methods for DNA isolation. A. Large scale double-stranded DNA isolation. The method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (17,18) followed by equilibrium ultracentrifugation in cesium chloride-ethidium ...  
 
 
 
PCR clean-up Gel extraction - MACHEREY-NAGEL Homepage 
 
www.mn-net.com/Portals/8/attachments/.../DNA%20clean-up/...
Single stranded DNA clean-up 1 PCR clean-up, DNA clean-up, or single stranded DNA clean-up: Adjust binding condition Gel extraction: Excise DNA fragment / solubilize gel slice 200 μL NTI/ ... during DNA gel extraction. 12 11 10 9 8 5 4 7 6 pH yellow blue green  
 
 
 
DNA EXTRACTION METHODS 
 
xa.yimg.com/.../24240295/21032036/name/DNAExtraction.ppt
DNA EXTRACTION METHODS What are the essential components of a DNA extraction Procedure? Maximize DNA recovery Remove inhibitors Remove or inhibit nucleases Maximize the quality of DNA Double strand vs. Single strand (RFLP or PCR) How Much DNA Can We Recover?  
 
 
 
Purification and Analysis of Autoimmune Antibody Reactive ... 
 
books.google.com/books/about/Purification_and_Analysis...
Purification and Analysis of Autoimmune Antibody Reactive with Single Stranded DNA.  
 
 
 
Gone Fishin' : Labsystems' KingFisher(TM) Automates ... 
 
www.the-scientist.com/?articles.view/articleNo/19531
Gone Fishin' : Labsystems' KingFisher(TM) Automates Purification of Single-Stranded DNA. In the rush to sequence every gene in existence researchers need to increase their capacity and throughput.  
 
 
 
Single Stranded Templates for Pyrosequencing™ 
 
www.b2b.invitrogen.com/site/us/en/home/Products-and...
Pyrosequencing™ requires a single stranded sequencing template. The simplest way to prepare this is by the use of Dynabeads M-280 Streptavidin . First the DNA fragment of interest is PCR amplified with one of the primers biotinylated.  
 
 
 
FosmidMAX™ DNA Purification Kit - Epicentre (an Illumina ... 
 
www.epibio.com/.../fosmidmax-dna-purification-kit
Single-Stranded DNA Binding Protein (SSB), E. coli; Ligases, Kinases, & Phosphatases. DNA Ligases. T4 DNA Ligase, Cloned; Ampligase® Thermostable DNA Ligase; ... FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA.  
 
 
 
M13mp18 Single-stranded DNA | NEB 
 
https://www.neb.com/.../n4040-m13mp18-single-stranded-dna
Is the M13mp18 Single-Stranded DNA circular or linear? Datacards; Datacards The ... The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed! Products. All; Restriction Endonucleases; PCR, ...  
 
 
 
Fluorescent DNA probes for ultra-sensitive strand-specific ... 
 
www.nature.com/protocolexchange/protocols/2802
We have developed a method to synthesize strand-specific, fluorescent DNA probes that bind defined genomic sequences. PCR with biotin-labelled forward primers and natural reverse primers permits purification of the individual strands; incorporation of aminoallyl-dUTP results in exposed amino ...  
 
 
 
Single-Stranded DNA Binding Proteins - Microbiology ... 
 
microbiology.ucdavis.edu/kowalczykowski/PDF_files/...
21. DNA BINDING PROTEINS 377 frequency to nucleate the transition to the single-stranded-protein- complexed form. As discussed in Section IV,A, such kinetic blocks may  
 
 
 
Polymerase chain reaction - Wikipedia, the free encyclopedia 
 
en.wikipedia.org/wiki/Pcr
The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.    
 
 
Isolation of SingleStranded DNA - Current Protocols 
 
www.currentprotocols.com/WileyCDA/CPUnit/refId-mb0215.html
Single‐stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies  
 
 
 
Family Shuffling with Single-Stranded DNA 
 
www.scs.illinois.edu/~zhaogrp/publications/HZ13.pdf
Family Shuffling with ssDNA 93 stranded DNA templates requires the subcloning of the target genes to a phagemid vector and the use of a helper phage.  
 
 
 
Tissue and Hair Extraction Kit (for use with DNA IQ™) Protocol 
 
www.promega.com/resources/protocols/technical-bulletins/...
A protocol for purifying double-stranded or single-stranded DNA from tissue and hair samples. This protocol includes details for purification of genomic and mitochondrial DNA from hair follicles and hair shafts and purification of genomic DNA from fresh, frozen, or formalin-fixed, paraffin ...  
 
 
 
Simple and effective method for generating single-stranded ... 
 
www.biotechniques.com/multimedia/archive/00002/BTN_A...
INTRODUCTION The creation of amplification methods to generate single-stranded DNA (1,2) has represented a major advance in development of PCR  
 
 
 
Protocol for Single Stranded DNA Preparation 
 
molecularstation.com/dna/dna-protocols/single-strand-dna...
Protocol for single stranded DNA preparation. Single Strand DNA Preparation Background. Single stranded DNA consists of one chain of nucleotides rather than the two base pairing strands found in the double stranded DNA form.  
 
 
 
Single-Stranded Conformation Polymorphism (SSCP) 
 
www.appliedbiosystems.com/absite/us/en/.../dna.../sscp.html
Single-stranded conformation polymorphism ... Single-stranded conformation polymorphism (SSCP) ... Step 1: DNA Purification ; Step 2: Primer Design & PCR Amplification; Step 3: Sample Prep for Fragment Analysis Run ; Step 4: Capillary Electrophoresis;  
 
 
 
Evaluation of DNA Extraction Efficiency - Material ... 
 
cstl.nist.gov/.../pub_pres/AAFS2013-Butts-DNAextraction.pdf
Applied Genetics Evaluation of DNA Extraction Efficiency Erica L.R. Butts, Jamie L. Almeida, Margaret C. Kline, Peter M. Vallone 65th American Academy of Forensic Sciences  
 
 
 
Mitochondrial Single-stranded DNA-binding Protein from ... 
 
www.jbc.org/content/270/36/21137.full
Abstract. Using a stringent purification procedure on single-stranded DNA cellulose, we have isolated the mitochondrial single-stranded DNA-binding protein from  
 
 
 
prepGEM™ DNA Extraction – Insect | TATAA Biocenter 
 
www.tataa.com/products-page/dna-extraction/prepgem-dna...
ZyGEM’s prepGEM™ DNA extraction technology, based on the proprietary EA1 enzyme, can extract high quality DNA from a broad range of sample materials in less than 20 minutes. ... prepGEM™ extracted DNA is largely single-stranded because of the heat step.  
 
 
 


No more results ...
Copyright © 2016 Umuwa. All Rights Reserved. About us / Privacy Policy / Sitemap Most searched