www.zymoresearch.com/rna/total-rna-purification/dna-rna... The ssDNA/RNA Clean & Concentrator™ provides a simple and reliable method for the rapid separation, clean-up, and concentration of up to ~5 µg (per prep.) of single stranded DNA and/or RNA from double stranded species (e.g., genomic DNA). This
www.biotechniques.com/BiotechniquesJournal/2005/May/... Single-stranded DNA (ssDNA) is very useful in diagnosis, molecular therapy, and basic research in molecular biology. When high-throughput or frequent preparation of ssDNA is needed, it is highly desirable to accomplish the task by a procedure that is rapid, efficient, cost-effective, and automation
www.sciencedirect.com/science/article/pii/0003269781905467 Several simple methods that aid in the high-yield purification of small, single-stranded DNA are described. The methods rely initially on the silicone coating o
www.lifetechnologies.com/us/en/home/life-science/dna-rna... Many applications require the use of single-stranded DNA templates. The immobilization of a double-stranded PCR product for preparation of two single-stranded DNA fragments is probably the most widely used protocol for Dynabeads® M-280 Streptavidin.
www.ncbi.nlm.nih.gov/pubmed/22047177 Preparation of single-stranded DNA from PCR products with streptavidin magnetic beads. Wilson R. ... DNA, Single-Stranded/isolation & purification* Immunomagnetic Separation/methods* Polymerase Chain Reaction; Sodium Hydroxide/chemistry*
www.sigmaaldrich.com/life-science/molecular-biology/dna... The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts.
www.b2b.invitrogen.com/site/us/en/home/Products-and... Many applications require the use of single-stranded DNA templates. The immobilisation of a double-stranded PCR product for preparation of two single-stranded DNA fragments is probably the most widely used protocol for Dynabeads® M-280 Streptavidin.
link.springer.com/article/10.1023/A%3A1008829931836 A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric
www.sciencedirect.com/science/article/pii/S0003269796900536 A solid-phase triple-helix-mediated affinity capture method is described for the purification of single-stranded M13 DNA for use as template in fluorescence-bas
clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17487 Single stranded DNA clean-up 1 PCR clean-up, DNA clean-up, or single stranded DNA clean-up: Adjust binding condition Gel extraction: ... purification of DNA from samples without SDS the standard protocol for PCR clean-up can be used (see section 5.1).
public.callutheran.edu/~revie/protocols/Q-Minipreps.pdf Purification of plasmid DNA Plasmids are small double-stranded DNA pieces of DNA that exist outside the chromosome of the cell in which they are located.
molecularstation.com/forum/dna-techniques/66216-single... Hi all, Did anyone do single stranded dna purification using streptavidin agarose beads before? I PCR amplified a single stranded dna library to get double stranded dna using reverse-biotinylated
en.wikipedia.org/wiki/M13_bacteriophage M13 is a filamentous bacteriophage composed of circular single stranded DNA which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
www.sjsu.edu/.../courses/c3/s0/DNA%20Extraction.ppt Chelex Extraction The Chelex extraction process denatures double stranded DNA and yields single stranded DNA, ... Different DNA extraction methods used in forensic DNA PowerPoint Presentation What are the essential components of a DNA extraction Procedure?
www.protocol-online.org/biology-forums/posts/11029.html Hi, I am not a biologist or biochemist and wonder if I cold get help with the following from an expert: I am trying to purify long (around 1 micrometer say) single stranded DNA, the aim is to get rid of shorter polimerization products primers and nucleotides.
www.ncbi.nlm.nih.gov/pmc/articles/PMC3814375 Single-stranded C18 cartridge DNA purification. The single-stranded oligonucleotides obtained from ~1 µmol syntheses were resuspended in TE buffer [10 mM Tris-HCl, 1 mM ethylenediamine tetraacetic acid (EDTA), pH 8.0] with 150 mM NaCl and purified using Waters C18 Sep-Pak cartridges.
www.piercenet.com/product/deoxyribonuclease-i-dnase-i Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA.
dharmacon.gelifesciences.com/.../single-strand-rna-synthesis... Single-stranded RNA can be shipped in its 2' protected form to improve stability. As a result of chemical RNA synthesis, all oligonucleotides will have chemical protecting groups at the 2' position of each nucleotide.
www.mn-net.com/tabid/1452/default.aspx • Separate buffers for single stranded DNA or SDS containing samples available ... • Clean-up of single stranded DNA and RNA extraction from agarose gels ... • NucleoSpin ® Gel and PCR Clean-up ensures complete removal of contaminations such as:
www.promega.com/.../readyamp-genomic-dna-purification-system... System for purification of single-stranded DNA from whole blood or blood stains. DNA may be used directly in amplification reactions without further manipulation. No organic extraction or ethanol precipitation.
www.sigmaaldrich.com/.../dna-and-rna-purification/... Post Reaction Cleanup in DNA & RNA Purification: Sigma-Aldrich provides GenElute™ PCR clean-up, agarose spin columns and minus EtBr spin columns for purification of single or double stranded PCR amplification fragments; as well as extraction of DNA fragments from Ethidium Bromide (EtBr ...
www.nature.com/nprot/journal/v8/n4/full/nprot.2013.038.html Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA. Marie-Theres Gansauge 1, Matthias Meyer 1, Affiliations; Contributions
www.jimmunol.org/content/125/2/874 Antibodies to double-stranded DNA: purification and characterization of binding specificities. A C Gilliam, ... that binds only to entirely double-stranded DNA and other types that bind to both double- and single-stranded DNA. We report also that ...
www.qiagen.com/.../dna-cleanup/qiaquick-pcr-purification-kit... The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.
www.lifetechnologies.com/us/en/home/life-science/dna-rna... Pyrosequencing™ requires a single stranded sequencing template. The simplest way to prepare this is by the use of Dynabeads M-280 Streptavidin . First the DNA fragment of interest is PCR amplified with one of the primers biotinylated.
www.genome.ou.edu/protocol_book/protocol_partIII.html III. Methods for DNA isolation. A. Large scale double-stranded DNA isolation. The method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (17,18) followed by equilibrium ultracentrifugation in cesium chloride-ethidium ...
www.mn-net.com/Portals/8/attachments/.../DNA%20clean-up/... Single stranded DNA clean-up 1 PCR clean-up, DNA clean-up, or single stranded DNA clean-up: Adjust binding condition Gel extraction: Excise DNA fragment / solubilize gel slice 200 μL NTI/ ... during DNA gel extraction. 12 11 10 9 8 5 4 7 6 pH yellow blue green
xa.yimg.com/.../24240295/21032036/name/DNAExtraction.ppt DNA EXTRACTION METHODS What are the essential components of a DNA extraction Procedure? Maximize DNA recovery Remove inhibitors Remove or inhibit nucleases Maximize the quality of DNA Double strand vs. Single strand (RFLP or PCR) How Much DNA Can We Recover?
www.the-scientist.com/?articles.view/articleNo/19531 Gone Fishin' : Labsystems' KingFisher(TM) Automates Purification of Single-Stranded DNA. In the rush to sequence every gene in existence researchers need to increase their capacity and throughput.
www.b2b.invitrogen.com/site/us/en/home/Products-and... Pyrosequencing™ requires a single stranded sequencing template. The simplest way to prepare this is by the use of Dynabeads M-280 Streptavidin . First the DNA fragment of interest is PCR amplified with one of the primers biotinylated.
www.epibio.com/.../fosmidmax-dna-purification-kit Single-Stranded DNA Binding Protein (SSB), E. coli; Ligases, Kinases, & Phosphatases. DNA Ligases. T4 DNA Ligase, Cloned; Ampligase® Thermostable DNA Ligase; ... FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA.
https://www.neb.com/.../n4040-m13mp18-single-stranded-dna Is the M13mp18 Single-Stranded DNA circular or linear? Datacards; Datacards The ... The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed! Products. All; Restriction Endonucleases; PCR, ...
www.nature.com/protocolexchange/protocols/2802 We have developed a method to synthesize strand-specific, fluorescent DNA probes that bind defined genomic sequences. PCR with biotin-labelled forward primers and natural reverse primers permits purification of the individual strands; incorporation of aminoallyl-dUTP results in exposed amino ...
microbiology.ucdavis.edu/kowalczykowski/PDF_files/... 21. DNA BINDING PROTEINS 377 frequency to nucleate the transition to the single-stranded-protein- complexed form. As discussed in Section IV,A, such kinetic blocks may
en.wikipedia.org/wiki/Pcr The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
www.scs.illinois.edu/~zhaogrp/publications/HZ13.pdf Family Shuffling with ssDNA 93 stranded DNA templates requires the subcloning of the target genes to a phagemid vector and the use of a helper phage.
www.promega.com/resources/protocols/technical-bulletins/... A protocol for purifying double-stranded or single-stranded DNA from tissue and hair samples. This protocol includes details for purification of genomic and mitochondrial DNA from hair follicles and hair shafts and purification of genomic DNA from fresh, frozen, or formalin-fixed, paraffin ...
molecularstation.com/dna/dna-protocols/single-strand-dna... Protocol for single stranded DNA preparation. Single Strand DNA Preparation Background. Single stranded DNA consists of one chain of nucleotides rather than the two base pairing strands found in the double stranded DNA form.
cstl.nist.gov/.../pub_pres/AAFS2013-Butts-DNAextraction.pdf Applied Genetics Evaluation of DNA Extraction Efficiency Erica L.R. Butts, Jamie L. Almeida, Margaret C. Kline, Peter M. Vallone 65th American Academy of Forensic Sciences
www.tataa.com/products-page/dna-extraction/prepgem-dna... ZyGEM’s prepGEM™ DNA extraction technology, based on the proprietary EA1 enzyme, can extract high quality DNA from a broad range of sample materials in less than 20 minutes. ... prepGEM™ extracted DNA is largely single-stranded because of the heat step.