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pRSFDuet™-1 - SnapGene | Software for everyday molecular ... 
 
www.snapgene.com/.../pRSFDuet-1
pRSFDuet™-1 Bacterial vector with an RSF 1030 origin for the co-expression of two genes. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. ... The map, notes, ...  
 
 
 
Nova gen - University of Utah Biochemistry Department of ... 
 
biochem.web.utah.edu/hill/links/pRSFDuet-1.pdf
pRSFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multi-ple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). ... Unique sites are shown on the circle map.  
 
 
 
DNASU Plasmid | Detailed Vector Information: pRSFDuet 
 
dnasu.org/DNASU/GetVectorDetail.do?vectorid=575
Vector Name: pRSFDuet: DyNA Vector Map. Powered by LabGenius. Synonyms: pRSFDuet-1 pRSFDuet; Sequencing Primer: Forward: Unknown  
 
 
 
pRSFDuet-1 - Addgene Vector Database (Plasmids, Expression ... 
 
www.addgene.org/vector-database/4005
pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene. Catalog Number: 71340: Stable: Unspecified: Constitutive: ... Site Map; Addgene • One Kendall Sq B7102 • Cambridge MA 02139 • +1 (617) 225-9000 ...  
 
 
 
pRSFDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pRSFDuet-1
Notes. pRSFDuet-1 is designed for the coexpression of two target proteins. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs.  
 
 
 
pET & Duet Vectors (Novagen) - SnapGene | Software for ... 
 
www.snapgene.com/resources/plasmid__files/pet_and_duet...
These combined DNA sequence and map files can be opened with SnapGene or the free SnapGene Viewer. pET & Duet Vectors (Novagen).zip (2 MB) ... pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material.  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/prsfduet-1-dna,EMD...
pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) ... TB391 pRSFDuet™-1 Vector Map: Vector Sequence. Title; pRSFDuet™-1 Vector Sequence: Related Products & Applications Categories.  
 
 
 
pRSFDuet - 生物通--------中国生命科学第一网 
 
www.ebiotrade.com/buyf/productsf/Novagen/71341-3.htm
pRSFDuet-1 DNA: Cat. No. 71341-3: Product Description: pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by ... •TB391 pRSFDuet™-1 Vector Map  
 
 
 
pRSFDuet - LabGenius 
 
beta.labgeni.us/registries/DNASU/pRSFDuet
pRSFDuet. DNASU. Bacterial co ... Map Viewer. Cutters. All Single Cutters Double Cutters None. Show ORFs. Sequence; Parts; Oligos; Restriction Enzymes; Sequencing; Cancel Save. View Double Stranded Show ORFs. Cutters. All Single Cutters Double Cutters None.  
 
 
 
pRSF and pCDF Vectors - 生物通 ... 
 
www.ebiotrade.com/buyf/productsf/Novagen/tb401-000.pdf
pRSF and pCDF Vectors Table of Contents About ... PopCulture, pRSFDuet, rLysozyme, RoboPop, Rosetta, RosettaBlue, Rosetta-gami, SpinPrep, S•Tag, Trail Mix, ... Bulletins 392 and 393 for detailed maps of pCDF-1b and pRSF-1b, respectively).  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/prsfduet-1-dna,EMD...
pRSFDuet™-1 DNA. Cookies on the Merck Millipore Site. We use cookies on our website to help us to provide you with the best online experience possible. If you continue and accept all cookies, you will receive all of the cookies that we use on the Merck Millipore website.  
 
 
 
Protein Expression and Purification Facility (PEPF ... 
 
www.helmholtz-muenchen.de/en/pepf/materials/vector...
pRSFDuet-1: map: T7/lac: Kan: N-His: BL21 (DE3) RSF1030: Novagen: expression vector; co-expression of 2 target genes: pTrcHis2 A: map: trc/lac: Amp: C-His: BL21: pUC: Invitrogen: expression vector: pTrcHis2 B: map: trc/lac: Amp: C-His: BL21: pUC: Invitrogen: expression vector: pTrcHis2 C: map ...  
 
 
 
Structure of the AcrAB-TolC multidrug efflux pump : Nature ... 
 
www.nature.com/nature/journal/v509/n7501/full/nature...
Here we present the pseudo-atomic structure of a complete multidrug efflux pump in complex with a modulatory protein partner from E. coli. ... Electron density map with coefficients 2F o ... together with plasmid pRSFDuet-1-acrA-polyGlySer-acrZ His5-tolC ...  
 
 
 
pRSFDuet-1 pRSFDuet1载体质粒图谱、序列、价格 ... 
 
www.biofeng.com/zaiti/dachang/pRSFDuet-1.html
pRSFDuet1,pRSFDuet-1载体价格850元,pRSFDuet-1载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见下文,pRSFDuet1载体抗性为卡那霉素,质粒大小为3829 bp,测序引物为T7和T7t,含有N-His标签,Novagen公司出品,是大肠杆菌蛋白双表达 ...  
 
 
 
Escherichia coli expression vectors | my-whiteboard 
 
www.my-whiteboard.com/escherichia-coli-expression-vectors
Escherichia coli expression vectors Vector Vector map Promoter Antibiotic. Escherichia coli expression vectors. Escherichia coli expression vectors. Vector Vector map ... pRSFDuet-1: map: T7/lac: Kan: N-His : BL21 (DE3) RSF1030: Novagen: expression vector; co-expression of 2 target genes ...  
 
 
 
Intein-based biosynthetic incorporation of unlabeled protein ... 
 
www.nature.com/nbt/journal/v23/n6/fig_tab/nbt1097_F2.html
(a) Schematic map of pJJDuet30 for the expression of the N-terminal portion, derived from pRSFDuet-1 (Novagen). (b) Schematic map of pSFBAD09 for the expression of the C-terminal portion. This vector is derived from pBAD. See Methods for details  
 
 
 
pACYCDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pACYCDuet-1
This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.. History .  
 
 
 
pRSFDuet-1载体_质粒图谱 - 优宝生物 
 
www.youbio.cn/product/vt1238
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). ... Unique sites are shown on the circle map.    
 
 
Patent US7582475 - Vectors and methods for high throughput co ... 
 
www.google.com/patents/US7582475
FIG. 2 shows a map of the pDEST-C1 vector. ... and ligated into similarly digested pCDFDuet-1, pRSFDuet-1, and pETDuet-1 to create pRIPPER-1, pRIPPER-2, and pRIPPER-4 respectively. ... "Novagen: TB392: pCDF-1b Vector Map," 3 pages. 3 "Novagen: TB401: ...    
 
 
71340 | pCDFDuet™-1 DNA - Novagen 
 
www.merckmillipore.com/INTL/en/product/pCDFDuet%E2%84%A2...
... Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 combination; Lane9: pRSFDuet-1, pCDFDuet-1 ... TB390VM pCDFDuet™-1 Vector Map: Vector Sequence. Title; pCDFDuet™-1 Vector Sequence:  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/INTL/en/product/prsfduet-1-dna...
pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) ... TB391 pRSFDuet™-1 Vector Map: Secuencia vectorial. Cargo; pRSFDuet™-1 Vector Sequence: Productos y aplicaciones relacionados  
 
 
 
Nova gen 
 
www.yrbio.com/.../document/vector/226426/pcdfduet-1_map.pdf
pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ... Unique sites are shown on the circle map.  
 
 
 
Reconstitution of Active Mycobacterial Binuclear Iron ... 
 
aem.asm.org/content/79/19/6033.full.pdf
Reconstitution of Active Mycobacterial Binuclear Iron Monooxygenase Complex in Escherichia coli Toshiki Furuya, Mika Hayashi, Kuniki Kino DepartmentofAppliedChemistry,FacultyofScienceandEngineering,WasedaUniversity,Shinjuku-ku,Tokyo,Japan  
 
 
 
pRSFDuet-1 | 基因搜 
 
www.genesou.com/?product=prsfduet-1
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
Chris Hill Lab - Resources - University of Utah Biochemistry ... 
 
biochem.web.utah.edu/hill/resources.htm
Vector Maps; Primer design; Oligo Synthesis and Sequencing; Protein Expression ; Protien Purification ; Protein Analysis; ... DUET Vectors: pACYDuet-1, pCDFDuet-1, pCOLADuet-1, pRSFDuet-1, pETDuet-1; pET Vectors: pET16b, pET26b-TEV, pET14b, ...  
 
 
 
pRSFDuet-1 Vector_百度文库 
 
wenku.baidu.com/view/a3c74ca50029bd64783e2c19.html
Novagen pRSFDuet-1 Vector pRSFDuet-1 DNA TB391 0903 Cat. No. 71341-3 3582–3598 1 83–100 69–168 214–230 231 297 ... the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map. Pfo I (3766) Acl I (3674) BstAP I (3658 ...  
 
 
 
DNA binding to RecD: role of the 1B domain in SF1B helicase ... 
 
emboj.embopress.org/content/27/16/2222
It has been shown that disruption of the helicase activity in PcrA by mutations in the 1B and 2B domains uncoupled helicase from translocase ... pRSFDuet‐recC and pCDFDuet ... The map calculated from the refined structure was used to manually rebuild large changes in protein structure and ...  
 
 
 
Supporting Information - PNAS 
 
www.pnas.org/content/suppl/2012/10/11/1215241109.DC...
oned into pRSFDuet vector (Novagen) and overexpressed in Rosetta 2 (DE3) cells (Novagen). ... (3D-MAP) and 1× protease in-hibitor mixture]. After clearing the cellular debris (12,250 × g, 60 min at 4 °C) the lysate was slowly brought to 100 mM am-  
 
 
 
Flexible tethering of primase and DNA Pol in the eukaryotic ... 
 
nar.oxfordjournals.org/content/39/18/8187.full.pdf
pRSFDuet-1 vector expressing full-length PriS and His-tagged PriL (49–513). The first 48 amino acids of PriL were omitted as they are not conserved and are likely to ... map between the primosome and the Pol a–B subunit complex (12) ...  
 
 
 
Patent US20120244588 - Method of producing 3-hydroxypropionic ... 
 
www.google.com/patents/US20120244588
Method of producing 3-hydroxypropionic acid using malonic semialdehyde reducing pathway US 20120244588 A1. ... pRSFDuet-1 (EMD Chemicals), was ... Maps of each of the recombinant vectors disclosed herein are illustrated in FIG. 4: (1) ...  
 
 
 
EMDB-5127: Three-dimensional EM structure of an intact ... 
 
pdbj.org/emnavi/emnavi_detail.php?id=5127
Three-dimensional EM structure of an intact activator-dependent transcription initiation complex: Map: E. coli Class I transcription activation complex: ... Vector: pEcABC-H6, pRSFduet-sigma, pCDF-omega NCBI taxonomy: 469008 Expression system: ...  
 
 
 
An RNA Degradation Machine Sculpted by Ro Autoantigen and ... 
 
www.sciencedirect.com/science/article/pii/S0092867413002699
Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs ... The difference maps between each pair of images are shown in the ... we cloned Rsr containing an N-terminal His 6-tag and Strep-PNPase into pRSFDuet-1 (Novagen). In addition, Y ...  
 
 
 
AEM Accepts, published online ahead of print on 26 July 2013 ... 
 
aem.asm.org/content/early/2013/07/22/AEM.01856-13.full.pdf
Schematic maps of the 124 plasmids are shown in Fig. S1 in the supplemental material. Bacteria were grown in 125 Luria-Bertani (LB) ... pRSFDuet-1 Vector used for cloning, RSF origin, Kn r Novagen pCDFDuet-1 Vector used for cloning, CDF origin, ...  
 
 
 
Supporting Information - PNAS 
 
www.pnas.org/content/suppl/2011/11/10/1110951108.DC...
teins in Escherichia coli; pRSFDuet-1 (Novagen), which was modified for expression of proteins with an N-terminal hexahis- ... The 2Fo-Fc electron density map in the vicinity of Trp31 in the helix is shown in blue. (B)  
 
 
 
Reconstitution, Activities, and Structure of the Eukaryotic ... 
 
www.sciencedirect.com/science/article/pii/S0092867406014279
While C230Y occurs in a hydrophobic core, many other mutations map to the bottom of the PH-domain ring, ... hRRP42 and hMTR3 were inserted into pRSFDuet-1 to encode His 6-hRrp42/Mtr3. hRrp4, hRrp40, or hCsl4 were expressed as Nt His 6-fusions in pRSFDuet-1.  
 
 
 
Assembly and Architecture of Biogenesis of Lysosome-related ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3285357
Map Viewer; Online Mendelian Inheritance in Man (OMIM) RefSeqGene; UniGene; All Genes & Expression Resources... Genetics & Medicine. Bookshelf; Database of Genotypes and Phenotypes (dbGaP) ... , and the product of BLOS1 was cloned into the pRSFDuet-1 vector (Novagen).  
 
 
 
Loss of SUMO1 in mice affects RanGAP1 localization and ... 
 
jcs.biologists.org/content/121/24/4106.long
Loss of SUMO1 in mice affects RanGAP1 localization and formation of PML nuclear bodies, ... we also derived mice from RRQ016 ES cells. We were able to map the vector insertion ... cells transformed with pRSFDuet expressing either full-length or truncated His-tagged SUMO1 were grown ...  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/RO/en/product/prsfduet-1-dna,EMD...
Hier finden Sie die Produkte und Dienstleistungen für Ihren Prozess.  
 
 
 
PLOS Pathogens: Structure of a Bimodular Botulinum Neurotoxin ... 
 
journals.plos.org/plospathogens/article?id=10.1371/...
The M-PTC moiety was clearly identified in the EM density map based on its crystal structure . ... pRSFDuet-1, and pET28a, respectively. In addition, HA17 and HA33 were cloned into the bicistronic pRSFDuet-1 for co-expression. ...  
 
 
 
Crystal Structure of Get4-Get5 Complex and Its Interactions ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC2843242
... Get4 and Get5 simultaneously, the coding sequences of GET4 and GET5 were inserted into MCS2 and MCS1 of the vector pRSFDuet-1 (Novagen), respectively. The plasmid ... XtalView was used for examining electron density maps and manually model building.  
 
 
 
71146 | pETDuet™-1 DNA 
 
www.merckmillipore.com/INTL/en/product/petduet-1-dna,EMD...
Vector Map; Vector Sequence; 71146 ... This vector can be transformed into the same cell with pACYCDuet™-1, pCDFDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 in the appropriate host strain for the coexpression of up to eight target genes.  
 
 
 
Supplemental Information 091209 - Genes & Development 
 
genesdev.cshlp.org/content/suppl/2010/01/08/24.3.235.DC1/......
Supplemental information page. 1 ... 145-411, 194-411, 215-411 and 232-411) were subcloned into pRSFDuet-1 and pGEX-6P-1. DNA fragments encoding human YAP2 with varying truncations ... Electron density (2Fo-Fc) map covering the YAP ...  
 
 
 
Structure of DNMT1-DNA Complex Reveals a Role for ... 
 
www.sciencemag.org/content/suppl/2010/12/13/science...
autoinhibition in maintenance DNA methylation _____ Jikui Song1, Olga Rechkoblit1, Timothy H. Bestor2 and ... modified pRSFDuet-1 vector (Novagen), ... The FoFc map that resulted from molecular - replacement clearly revealed a translocation of DNA -DNA complex, in the hDNMT1  
 
 
 
Patent WO2012067220A1 - Method for expressing useful protein ... 
 
www.google.com/patents/WO2012067220A1?cl=en
Maps; Play; YouTube; News; Gmail; Drive; More. Calendar; Translate; Mobile; Books; Wallet; Shopping; Blogger; Finance; Photos; Videos; Docs; ... I was used pRSFDuet of (Novagen) for the expression vector.  
 
 
 
Biovector质粒载体菌种细胞基因库 - Biovector,Inc ... 
 
biovector.blog.163.com
pRSFDuet-1: Novagen: 原核表达 ... Tags: Biovector Inc. plasmid vector to obtain quotes price price sale map sequence cleavage sites transformed construct Buy purchase order sequence map by microinjecting the plasmid containing vector gene strain order resistance restriction site.    
 
 
Engineering of recombinant E. coli cells co-expressing ... 
 
onlinelibrary.wiley.com/doi/10.1002/bit.24632/abstract
E. coli (P450pyrTM-GDH) with dual plasmids, pETDuet containing P450pyr triple mutant I83H/M305Q/A77S (P450pyrTM) and ferredoxin reductase (FdR) genes and pRSFDuet containing glucose dehydrogenase (GDH) and ferredoxin (Fdx) genes, was engineered to show a high activity (12.7 U g −1 cdw) for the ...  
 
 
 
PLOS ONE: Exploring the Electron Transfer Pathway in the ... 
 
www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal...
Two compatible plasmids, pRSET-b and pRSFDuet-1, were used for co-expressing cytochrome P450 gene cyp107z13, fd68 and fdr18/fdr28 in E. coli BL21(DE3). pRSET was used for expressing CYP107Z13, ... Map of the fd68 knock-out plasmids pKC1139:: fd68.  
 
 
 
Engineering of recombinant E. coli cells co-expressing ... 
 
www.sigmaaldrich.com/catalog/papers/22886996
... (P450pyrTM) and ferredoxin reductase (FdR) genes and pRSFDuet containing glucose dehydrogenase (GDH) and ferredoxin (Fdx) genes, was engineered to show a high activity (12.7 U g⁻¹ cdw) ... Site Map; Careers; Events; Programs; Reach Regulations; Contact Us; Literature Portal; Tool Box;  
 
 
 
Class I and II Lantibiotics from Geobacillus ... 
 
www.freepatentsonline.com/y2015/0050256.html
A multimeric geobacillin polypeptide can be a multiple antigen peptide (MAP). See e.g., Tam, J. Immunol. ... For geobacillin II production, GeoAII and GeoM were cloned in MCS1 and MCS2 of a pRSFDuet-1 vector, respectively.  
 
 
 
Flexible tethering of primase and DNA Pol α in the ... 
 
nar.oxfordjournals.org/content/early/2011/06/28/nar.gkr...
The yeast primase was produced in Rosetta 2 (DE3) Escherichia coli strain using the vector pRSFDuet-1 vector expressing full-length PriS and His-tagged PriL ... Indeed, a subtraction map between averages of the Prim lobe and the Prim complex after alignment revealed that, ...  
 
 
 


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