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pRSFDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pRSFDuet-1
Notes. pRSFDuet-1 is designed for the coexpression of two target proteins. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs.  
 
 
 
Nova gen - University of Utah Biochemistry Department of ... 
 
biochem.web.utah.edu/hill/links/pRSFDuet-1.pdf
pRSFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multi-ple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
pRSFDuet™-1 - SnapGene | Software for everyday molecular ... 
 
www.snapgene.com/.../pRSFDuet-1
pRSFDuet™-1 Bacterial vector with an RSF 1030 origin for the co-expression of two genes.  
 
 
 
pRSFDuet-1 - Addgene Vector Database (Plasmids, Expression ... 
 
www.addgene.org/vector-database/4005
Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/prsfduet-1-dna,EMD...
Description Overview pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/INTL/en/product/prsfduet-1-dna...
pRSFDuet™-1 DNA ... Free up your time and resources with Chromabolt® prepacked chromatography columns.  
 
 
 
Table 1 - Nature Methods - Nature Publishing Group : science ... 
 
www.nature.com/nmeth/journal/v3/n1/fig_tab/nmeth0106-55...
pRSFDuet-1: pCOLADuet-1: pMAL (all) tac: Ampicillin or carbenicillin: 50 g/ml: ColE1 (pBR322) pACYCDuet-1 [40] pCDFDuet-1: pRSFDuet-1: pCOLADuet-1: a Reduce the concentration of antibiotic used by half if transforming more than one plasmid. Next : Close window  
 
 
 
pRSFDuet-1 DNA 
 
www.chemicalbook.com/ProdSupplierGWCB91037990_EN.htm
pRSFDuet-1 DNA Basic information More.. Product Name: pRSFDuet-1 DNA: Synonyms: pRSFDuet-1 DNA: CAS: MF: MW: 0: EINECS: Mol File: Mol File: Information Error Report: Your Email: Browse by Nationality pRSFDuet-1 DNA Suppliers: China suppliers:  
 
 
 
DNASU Plasmid | Detailed Vector Information: pRSFDuet 
 
dnasu.org/DNASU/GetVectorDetail.do?vectorid=575
Vector Name: pRSFDuet: DyNA Vector Map. Powered by LabGenius. Synonyms: pRSFDuet-1 pRSFDuet; Sequencing Primer: Forward: Unknown  
 
 
 
pRSFDuet-1 DNA 
 
www.chemicalbook.com/ChemicalProductProperty_EN_CB...
Visit ChemicalBook To find more pRSFDuet-1 DNA information like chemical properties,Structure,melting point,boiling point,density,molecular formula,molecular weight, physical properties,toxicity information,customs codes. You can also browse global suppliers,vendor,prices,Price,manufacturers of ...  
 
 
 
Supplementary Table 1: Plasmids used in this study 
 
www.jbc.org/content/suppl/2011/10/17/M111.264028.DC1/jbc...
Supplementary Table 1: Plasmids used in this study . Plasmids Description Reference  
 
 
 
71340 | pCDFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/pcdfduet-1-dna,EMD...
描述 概述 pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 
pRSFDuet-1 pRSFDuet1载体质粒图谱、序列、价格 ... 
 
www.biofeng.com/zaiti/dachang/pRSFDuet-1.html
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
pRSFDuet-1载体_质粒图谱 - 优宝生物 
 
www.youbio.cn/product/vt1238
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).    
 
 
pRSFDuet-1 DNA - 生物通--------中国生命科学第一网 
 
www.ebiotrade.com/buyf/productsf/Novagen/71341-3.htm
pRSFDuet-1 DNA: Cat. No. 71341-3: Product Description: pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding ...  
 
 
 
pET & Duet Vectors (Novagen) - SnapGene | Software for ... 
 
www.snapgene.com/resources/plasmid__files/pet_and_duet...
pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources".  
 
 
 
Co-expression with pETDuet-1 (Duet Expression System) From ... 
 
www.biocompare.com/Product-Reviews/40993-Co-expression...
Two kanamycin-resistant Duet vectors are available: pRSFDuet-1 carries the RSF1030 replicon and pCOLADuet-1 carries the ColA replicon. Each vector carries two expression units each controlled by a T7 lac promoter for high-level protein expression.  
 
 
 
pRSFDuet-1 Vector_百度文库 
 
wenku.baidu.com/view/a3c74ca50029bd64783e2c19.html
Novagen pRSFDuet-1 Vector pRSFDuet-1 DNA TB391 0903 Cat. No. 71341-3 3582–3598 1 83–100 69–168 214–230 231 297–438 366–410 462–509 742–1554 1662–2411 2610–3689 pRSFDuet-1 sequence landmarks T7 promoter-1 T7 transcription start-1 His?Tag? coding sequence Multiple cloning sites ...  
 
 
 
Strategies for protein coexpression in Escherichia coli 
 
joshua-torlab.cshl.edu/pdf/Tolia-Joshua-Tor-NatMeth.pdf
pRSFDuet-1 and/or pCOLADuet-1 (Novagen) Additional vectors: pGEX (any, Amersham Biosciences), pMAL (any, New England BioLabs) EQUIPMENT ... Coexpression of pGEX-4T-1 harboring the gene encoding GST-hAgo2 with pRSF-Duet  
 
 
 
Supporting Information 
 
www.pnas.org/content/suppl/2012/03/13/1116815109.DC...
pRSFDuet-1 (GeoAI/GeoB) and pACYCDuet-1 (GeoC) were picked and grown in LB media overnight. The culture was used to inoculate a 6-L culture in Terrific Broth media containing 50 mg∕L kanamycin and 34 mg∕L chloramphenicol.  
 
 
 
pRSFDuet-1 | 基因搜 
 
www.genesou.com/?product=prsfduet-1
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/INTL/en/product/prsfduet-1-dna...
Descripción Visión de conjunto pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 
The sequence of the enterococcal cytolysin imparts unusual ... 
 
www.nature.com/nchembio/journal/v9/n3/full/nchembio.1162...
The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry. Weixin Tang 1, 2, Wilfred A van der Donk 1, 2, Affiliations; Contributions; ... Each precursor peptide was expressed as an N-terminal His 6-tagged fusion peptide together with untagged CylM from a pRSFDuet-1 ...  
 
 
 
pRSFDuet-1 载体 | 质粒图谱 | vector - YRGene 
 
www.yrgene.com/product/vector/15641
pRSFDuet-1. 型号 载体名称 出品公司 载体用途 价格 Delivery ; VYN0196 : pRSFDuet-1: Novagen : 原核表达载体: 1000 : 现货 ...  
 
 
 
www.plosone.org 
 
www.plosone.org/article/fetchSingleRepresentation.action?......
pET-19b E. coli expression vector, Ampr Novagen pRSFDuet-1 E. coli dual-expression vector, Kmr Novagen pMAL-c4E E. coli expression vector, Ampr Novagen pMG103 E. coli – Mycobacterium shuttle vector, integrating, with amidase promoter, ...  
 
 
 
pRSFDuet-1pRSFDuet1载体价格1000元【365128导购 ... 
 
3413.365128.com/user/laqcasi/33.html
我们出售pRSFDuet-1载体质粒,pRSFDuet1载体价格报价为1000元,保证质量,请有需要pRSFDuet-1载体的朋友,联系我的手机 I47-825O-882O,请尽量短消息和我联系,我一般不接陌生人的电话,谢谢。  
 
 
 
Multivariate modular metabolic engineering of Escherichia ... 
 
www.sciencedirect.com/science/article/pii/S0168165613003362
Hence, it is supposed that these should be caused by that the pRSFDuet-1, which had high copy number, resulted in the negative effect on cell physiology and an increase in the metabolic burden of host cells.  
 
 
 
July - University of Kansas Medical Center - JARID1A 
 
guide5.net/j/july---university-of-kansas-medical-center...
JARID1A-PHD3 was fused to the C terminus of a His(63)-SUMO tag in a modified pRSFDuet-1 vector (Novagen), with a ubiquitin-like-protease (ULP)  
 
 
 
Strict regulation of gene expression from a high-copy plasmid ... 
 
faculty.baruch.cuny.edu/dgruber/Research_files/gruber...
of pRSFDuet-1, two, and three copies of the lacIq gene were in-serted into the vector (R6 and R7, respectively). R0 serves as a neg-ative control since it lacks a functional lacI gene and contains no operators. Monitoring gene expression in E. coli  
 
 
 
Number of cells in colony - Bacteria Escherichia coli - BNID ... 
 
bionumbers.hms.harvard.edu/bionumber.aspx?&id=104458&...
One can then calculate backwards to estimate the number of cells in the colony. If one just wants an average number of cells per colony, one could pick up about 100 colonies, suspend them all together, count cells, and divide the final number by 100.  
 
 
 
Electronic Supporting Information for Heterologous Production ... 
 
www.rsc.org/suppdata/cc/c2/c2cc36336d/c2cc36336d.pdf
of the pRSFDuet-1 vector between the NdeI and KpnI restriction sites. The garA gene was cloned by PCR from A. garbadinensis ATCC 31049 genomic DNA and inserted between the EcoRI and HindIII restriction sites of MCS1 in pRSFDuet-1.  
 
 
 
Efficient production of biodiesel from waste grease: One-pot ... 
 
www.sciencedirect.com/science/article/pii/S0960852412011571
Abstract. A novel concept and efficient method for producing biodiesel (FAME) from grease (15–40 wt% free fatty acid, FFA) were developed by using tandem lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system.  
 
 
 
AEM Accepts, published online ahead of print on 7 March 2014 ... 
 
aem.asm.org/content/early/2014/03/03/AEM.03986-13.full.pdf
94 pRSFDuet-1 and pCDFDuet-1 expression vectors were obtained from Novagen 95 (Darmstadt, Germany). All of the four Duet expression vectors were designed for the 96 coexpression of two target open reading frames (ORFs). One vector contains two  
 
 
 
pET载体 pRSFduet-1,pColAduet-1,pET His-上海北诺 ... 
 
www.chem17.com/st247307/product_11105287.html
上海北诺生物科技有限公司专业生产(供应)销售pET载体 pRSFduet-1,pColAduet-1,pET His系列产品,公司具有良好的市场信誉,专业的销售和技术服务团队,凭着经营pET载体 pRSFduet-1,pColAduet-1,pET His系列多年经验,熟悉并了解pET载体 ...  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/RO/en/product/prsfduet-1-dna,EMD...
Hier finden Sie die Produkte und Dienstleistungen für Ihren Prozess.  
 
 
 
pRSFDuet-1 pRSFDuet1载体质粒图谱,序列,价格,抗性 ... 
 
www.bbioo.com/experiment/104-59252-1.html
pRSFDuet1载体质粒图谱和多克隆位点信息. pRSFDuet1载体简介. pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
pACYCDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pACYCDuet-1
Notes. This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.  
 
 
 
Production of (R)-3-Quinuclidinol by E. coli Biocatalysts ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3497340
2.1. Construction of Expression Vector of QNR and LSADH. pET28a (Merck KGaA, Darmstadt, Germany) and pRSFDuet-1 (Merck) vectors have the kanamycin resistance gene.  
 
 
 
71340 | pCDFDuet™-1 DNA - Novagen 
 
www.merckmillipore.com/INTL/en/product/pCDFDuet%E2%84%A2...
pCDFDuet™-1 DNA - Novagen ... Description Overview pCDFDuet™-1 is designed for the coexpression of two target genes.  
 
 
 
PLOS ONE: Boost Protein Expression through Co-Expression of ... 
 
www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal...
pRSFDuet-1 (Novagen) was used for the co-expression target proteins (GFP, CAT, GUS, and HFBII) and the LEA-like peptide. HFBII is expressed as a fusion protein with GFP for readily evaluation of the protein expression.  
 
 
 
pRSFDuet-1载体 | pRSFDuet-1质粒图谱 
 
www.yrbio.com/bioresource/vector/prsfduet-1
pRSFDuet-1 : Novagen : 原核表达载体(双启动子) ¥1,000: 现货 : 质粒图谱 ...  
 
 
 
pCOLADuet 1 - VADLO - Life Sciences Search Engine 
 
www.vadlo.com/b/q?rel=0&keys=pCOLADuet+1
and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the ... EcoliWiki, EcoliHub. 30k cache View all results from this domain ...  
 
 
 
The Sequence of the Enterococcal Cytolysin Imparts Unusual ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3578037
The enterococcal cytolysin is a two-component lantibiotic of unknown structure with hemolytic activity that is important for virulence. ... Co-expression vector pRSFDuet-1 was obtained from Novagen. Construction of pRSFDuet-1 derivatives for co-expression of CylM and CylL L /CylL S.  
 
 
 
Nova gen - University of Utah Biochemistry Department of ... 
 
biochem.web.utah.edu/hill/links/pCDFDuet-1.pdf
pCDFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multi- ... pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins.  
 
 
 
The anti-activator ExsD forms a 1:1 complex with ExsA to ... 
 
www.jbc.org/content/suppl/2009/04/15/M109.003533.DC1/jbc...
1 The anti-activator ExsD forms a 1:1 complex with ExsA to inhibit transcription of type III secretion operons Julie Thibault, ... pRSFDuet-1 pACYCDuet pSE102 pRK-pC+lacZ pIApX 2 pIApX 2ExsD pRSF-pX 2ExsA pET15b-HExsA pET22b-ExsAH pET15b-HNter pET15b-HCter  
 
 
 
Team:SJTU-BioX-Shanghai/Project/project1.1 - 2012.igem.org 
 
2012.igem.org/Team:SJTU-BioX-Shanghai/Project/project1.1
Reconstructed Vectors. Vectors used in the project for membrane protein expression are modified versions of pRSFDuet-1, pETDuet-1,pACYCDuet-1(NOVAGEN), which are originally regulated by T7 promoter.  
 
 
 
Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable ... 
 
homepages.rpi.edu/~koffam/papers/2012_Malla_Koffas.pdf
Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable Flavonoid, in Escherichia coli ... pETDuet-1, pCDFDuet-1, pRSFDuet-1 (Novagen), and pET-24ma( ), donated by Hiroshi Sakamoto (Pasteur Institute, France), were used for cloning and subcloning.  
 
 
 
pET载体 pRSFduet-1,pColAduet-1,pET His-上海北诺 ... 
 
www.bnbiotech.com/beinuobio-Products-11105287
上海北诺生物科技有限公司专业供应销售pET载体 pRSFduet-1,pColAduet-1,pET His,欢迎您来电咨询pET载体 pRSFduet-1,pColAduet-1,pET His的详细信息!上海北诺生物科技有限公司提供的pET载体 pRSFduet-1,pColAduet-1,pET His不仅具有国内外 ...  
 
 
 
Structure of the Bifunctional Acyltransferase/Decarboxylase ... 
 
www.scripps.edu/shen/pdfs/176.pdf
Structure of the Bifunctional Acyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity ... The pRSFDuet-1 plasmid and Escherichia coli NovaBlue and BL21(DE3) cells were from Novagen (Madison, WI). Primer synthesis and DNA Figure 1.  
 
 
 
TB340 Duet Vectors RevD0304 
 
www.researchgate.net/publictopics.PublicPostFileLoader...
cloned into pRSFDuet-1 were substantially reduced in coexpression experiments with pETDuet-1 and pACYCDuet-1 in the same cell. A significantly different pattern of expression was obtained when the lower copy number pCOLADuet™-1 was substituted for pRSFDuet-1 in an otherwise  
 
 
 


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