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pRSFDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pRSFDuet-1
Notes. pRSFDuet-1 is designed for the coexpression of two target proteins. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs.  
 
 
 
Nova gen - University of Utah Biochemistry Department of ... 
 
biochem.web.utah.edu/hill/links/pRSFDuet-1.pdf
pRSFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multi-ple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
pRSFDuet™-1 - SnapGene | Software for everyday molecular ... 
 
www.snapgene.com/.../pRSFDuet-1
pRSFDuet™-1 Bacterial vector with an RSF 1030 origin for the co-expression of two genes.  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/prsfduet-1-dna,EMD...
Description Overview pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 
pRSFDuet-1 - Addgene Vector Database (Plasmids, Expression ... 
 
www.addgene.org/vector-database/4005
Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.  
 
 
 
DNASU Plasmid | Detailed Vector Information: pRSFDuet 
 
dnasu.org/DNASU/GetVectorDetail.do?vectorid=575
Vector Name: pRSFDuet: DyNA Vector Map. Powered by LabGenius. Synonyms: pRSFDuet-1 pRSFDuet; Sequencing Primer: Forward: Unknown  
 
 
 
Vector Database - Addgene - Homepage 
 
www.addgene.org/vector-database/query/?q_vdb=pRSFDuet-1
Name Vector Type Resistance Marker Bacterial Resistance Source Sequence; pRSFDuet-1: Bacterial Expression, coexpression: Kanamycin: EMD Biosciences |« « » »|  
 
 
 
Efficient production of biodiesel from waste grease: One-pot ... 
 
www.sciencedirect.com/science/article/pii/S0960852412011571
They were inserted into the corresponding restriction enzyme sites of plasmid pRSFDuet to give recombinant expression plasmid pRSFDuet-Calb and pRSFDuet-Tll, respectively.  
 
 
 
Strategies for protein coexpression in Escherichia coli 
 
joshua-torlab.cshl.edu/pdf/Tolia-Joshua-Tor-NatMeth.pdf
Strategies for protein coexpression in Escherichia coli Niraj H Tolia & Leemor Joshua-Tor Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, ... pRSFDuet-1 and/or pCOLADuet-1 (Novagen) Additional vectors: pGEX (any, Amersham Biosciences), pMAL (any, New England BioLabs)  
 
 
 
Table 1 - Nature Methods - Nature Publishing Group : science ... 
 
www.nature.com/nmeth/journal/v3/n1/fig_tab/nmeth0106-55...
pRSFDuet-1: pCOLADuet-1: pMAL (all) tac: Ampicillin or carbenicillin: 50 g/ml: ColE1 (pBR322) pACYCDuet-1 [40] pCDFDuet-1: pRSFDuet-1: pCOLADuet-1: a Reduce the concentration of antibiotic used by half if transforming more than one plasmid. Next : Close window  
 
 
 
pET & Duet Vectors ( Novagen ) - SnapGene | Software for ... 
 
www.snapgene.com/resources/plasmid__files/pet_and_duet...
pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources".  
 
 
 
Multivariate modular metabolic engineering of Escherichia ... 
 
www.sciencedirect.com/science/article/pii/S0168165613003362
Hence, it is supposed that these should be caused by that the pRSFDuet-1, which had high copy number, resulted in the negative effect on cell physiology and an increase in the metabolic burden of host cells.  
 
 
 
The sequence of the enterococcal cytolysin imparts unusual ... 
 
www.nature.com/nchembio/journal/v9/n3/full/nchembio.1162...
The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry. Weixin Tang 1, 2, Wilfred A van der Donk 1, 2, Affiliations; Contributions; ... Each precursor peptide was expressed as an N-terminal His 6-tagged fusion peptide together with untagged CylM from a pRSFDuet-1 ...  
 
 
 
Co-expression with pETDuet-1 (Duet Expression System) From ... 
 
www.biocompare.com/Product-Reviews/...with-pETDuet-1-Duet......
Two kanamycin-resistant Duet vectors are available: pRSFDuet-1 carries the RSF1030 replicon and pCOLADuet-1 carries the ColA replicon. Each vector carries two expression units each controlled by a T7 lac promoter for high-level protein expression.  
 
 
 
pRSFDuet-1 DNA - 生物通--------中国生命科学第一网 
 
www.ebiotrade.com/buyf/productsf/Novagen/71341-3.htm
pRSFDuet-1 DNA: Cat. No. 71341-3: Product Description: pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding ...  
 
 
 
The Sequence of the Enterococcal Cytolysin Imparts Unusual ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3578037
The enterococcal cytolysin is a two-component lantibiotic of unknown structure with hemolytic activity that is important for virulence. ... Co-expression vector pRSFDuet-1 was obtained from Novagen. Construction of pRSFDuet-1 derivatives for co-expression of CylM and CylL L /CylL S.  
 
 
 
Number of cells in colony - Bacteria Escherichia coli - BNID ... 
 
bionumbers.hms.harvard.edu/bionumber.aspx?&id=104458&...
ID: 104458: Property: Number of cells in colony Organism: Bacteria Escherichia coli: Value: 3.3e+9 Units  
 
 
 
Electronic Supporting Information for Heterologous Production ... 
 
www.rsc.org/suppdata/cc/c2/c2cc36336d/c2cc36336d.pdf
3 Overexpression and purification of His6-tagged GarM-modified GarA and GarA-V15L/I16V/19insK E. coli BL21 (DE3) cells transformed with a pRSFDuet-1 vector carrying the genes encoding GarM  
 
 
 
Strict regulation of gene expression from a high-copy plasmid ... 
 
faculty.baruch.cuny.edu/dgruber/Research_files/gruber...
of pRSFDuet-1, two, and three copies of the lacIq gene were in-serted into the vector (R6 and R7, respectively). R0 serves as a neg-ative control since it lacks a functional lacI gene and contains no operators. Monitoring gene expression in E. coli  
 
 
 
Production of (R)-3-Quinuclidinol by E. coli Biocatalysts ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3497340
Production of (R)-3-Quinuclidinol by E. coli Biocatalysts Possessing NADH-Dependent 3-Quinuclidinone Reductase (QNR or bacC) from Microbacterium luteolum and Leifsonia Alcohol Dehydrogenase (LSADH)  
 
 
 
Structure of the Bifunctional Acyltransferase/Decarboxylase ... 
 
www.scripps.edu/shen/pdfs/176.pdf
Structure of the Bifunctional Acyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity ... The pRSFDuet-1 plasmid and Escherichia coli NovaBlue and BL21(DE3) cells were from Novagen (Madison, WI). Primer synthesis and DNA Figure 1.    
 
 
TB340 Duet Vectors RevD0304 
 
www.researchgate.net/publictopics.PublicPostFileLoader...
cloned into pRSFDuet-1 were substantially reduced in coexpression experiments with pETDuet-1 and pACYCDuet-1 in the same cell. A significantly different pattern of expression was obtained when the lower copy number pCOLADuet™-1 was substituted for pRSFDuet-1 in an otherwise  
 
 
 
www.plosone.org 
 
www.plosone.org/article/fetchSingleRepresentation.action?......
pET-19b E. coli expression vector, Ampr Novagen pRSFDuet-1 E. coli dual-expression vector, Kmr Novagen pMAL-c4E E. coli expression vector, Ampr Novagen pMG103 E. coli – Mycobacterium shuttle vector, integrating, with amidase promoter, ...  
 
 
 
July - University of Kansas Medical Center - JARID1A 
 
guide5.net/j/july---university-of-kansas-medical-center...
JARID1A-PHD3 was fused to the C terminus of a His(63)-SUMO tag in a modified pRSFDuet-1 vector (Novagen), with a ubiquitin-like-protease (ULP)  
 
 
 
pACYCDuet-1 - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pACYCDuet-1
This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.. History .  
 
 
 
Team:SJTU-BioX-Shanghai/Project/project1.1 - 2012.igem.org 
 
2012.igem.org/Team:SJTU-BioX-Shanghai/Project/project1.1
Reconstructed Vectors. Vectors used in the project for membrane protein expression are modified versions of pRSFDuet-1, pETDuet-1,pACYCDuet-1(NOVAGEN), which are originally regulated by T7 promoter.  
 
 
 
Biotechnology for Biofuels | Full text | Assembly of lipase ... 
 
www.biotechnologyforbiofuels.com/content/8/1/34
The plasmids pACYCDuet-1, pRSFDuet-1, and pET-22(b) were obtained from Novagen (Darmstadt, Germany). Recombinant plasmid pET-28(b)-oleT JE for expression of the P450 fatty acid decarboxylase OleT JE was constructed in our laboratory previously . E. coli ...  
 
 
 
71340 | pCDFDuet™-1 DNA - Novagen 
 
www.merckmillipore.com/INTL/en/product/pCDFDuet%E2%84%A2...
... β-gal + Fluc; Lane2: pETDuet-1:GST/GUS + GFP; Lane3: pRSFDuet-1:Nus/hlFNγ + S•Tag/T4 PNK; Lane4: pCDFDuet-1:GUS + His•Tag/MBP; Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 ...  
 
 
 
The sequence of the enterococcal cytolysin imparts unusual ... 
 
www.researchgate.net/publication/234124056_The_sequence...
Publication » The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry..  
 
 
 
pRSF and pCDF Vectors - 生物通 ... 
 
www.ebiotrade.com/buyf/productsf/Novagen/tb401-000.pdf
pCDF-1b (SmRSpR) pRSF-1b or pRSFDuet-1 (KnR) 2–3 Group B pCDF-1b (SmRSpR) pACYCDuet-1 (CmR) 2–3 Group A Ap: ampicillin/carbenicillin, Kn: kanamycin, Cm: chloramphenicol, Sm: streptomycin, Sp: spectinomycin 4 Germany TB401 Rev. A 0903  
 
 
 
Histidine103inFra2IsanIron-SulfurClusterLigandinthe [2Fe-2S ... 
 
www.jbc.org/content/286/1/867.full.pdf
pRSFDuet-1-Fra1-His 6 /Fra2 1–35 plasmids were used for ex-pression and purification of apo-Fra2. As noted previously, Fra1-His 6 is not expressed at detectable levels from pRSF-Duet-1-Fra1-His 6 /Fra2 plasmids (9). Nevertheless, these co-  
 
 
 
Supplementary tables - Oxford Journals | Science ... 
 
abbs.oxfordjournals.org/content/suppl/2012/05/26/gms047...
pRSFDuet-1 T7lac promoter followed by two multiple cloning sites (MCS), Novagen. Carries the RSF1030 replicon, lacI and kanR . pRDUPBC pRSFDuet-1 with accPB cloned in EcoRV-XhoI sites this study. and accC cloned in BamHI-HindIII ...  
 
 
 
JCI - Platelet secretion and hemostasis require syntaxin ... 
 
www.jci.org/articles/view/75572/figure/1
STXBP5 is present in human platelets. (A) The indicated complexes were produced in E. coli using the pRSFDuet plasmid with the S-tag peptide fused to the C terminus of the syntaxin.  
 
 
 
RCSB Protein Data Bank - RCSB PDB - 4WVY Biology and ... 
 
www.rcsb.org/pdb/explore/biologyAndChemistry.do?...
4WVY: Structural Basis for Dodecameric Assembly States and Conformational Plasticity of the Full-Length AAA+ ATPases Rvb1.Rvb2  
 
 
 
Efficient Synthesis of Eriodictyol from L-Tyrosine in ... 
 
aem.asm.org/content/80/10/3072.full.pdf
Efficient Synthesis of Eriodictyol from L-Tyrosine in Escherichia coli Saijie Zhu, a,bJunjun Wu, ... pRSFDuet-1 Double T7 promoters, two MCS, RSF1030 origin; Knr Novagen pACYCDuet-1 Double T7 promoters, two MCS, p15A origin (pACYC184); Cmr Novagen  
 
 
 
Matthew Walsh | LinkedIn 
 
https://www.linkedin.com/pub/matthew-walsh/47/344/71
View Matthew Walsh's professional profile on LinkedIn. LinkedIn is the world's largest business network, helping professionals like Matthew Walsh discover inside connections to recommended job candidates, industry experts, and business partners.  
 
 
 
Supporting Information 
 
www.pnas.org/content/suppl/2012/03/13/1116815109.DC...
pRSFDuet-1 (GeoAI/GeoB) and pACYCDuet-1 (GeoC) were picked and grown in LB media overnight. The culture was used to inoculate a 6-L culture in Terrific Broth media containing 50 mg∕L kanamycin and 34 mg∕L chloramphenicol.  
 
 
 
pSKDuet01 from Addgene (Product Information, References, and ... 
 
www.lablife.org/p?a=products_show&id=19111
Alt Name/ID: 12172: Backbone: pRSFDuet, Novagen: Backbone Size: 3757: Gene/Insert: NpuDnaE: Alt Insert Name: DnaE N-intein, intein: Insert Size: 306: Species, Other: Nostoc punctiforme  
 
 
 
BiochemicalAnalysisoftheInteractionsbetweenthe ... 
 
www.jbc.org/content/287/43/36544.full.pdf
pRSFDuet-1/hydG Expression of untagged HydG protein pRSFDuet-1/hydG-6His Expression of a HydG protein with a N-terminal 6His tag pETDuet-1/hydA1-StrepII Expression of a HydA1 protein with a C-terminal StrepII tag  
 
 
 
Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable ... 
 
homepages.rpi.edu/~koffam/papers/2012_Malla_Koffas.pdf
Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable Flavonoid, in Escherichia coli ... pRSFDuet-1 Double T7 promoters; RSF ori Kmr Novagen pET24ma( r) Expression vector with T7 promoter; p15A ori Km Pasteur Institute, France  
 
 
 
Production of (R)-3-Quinuclidinol by E. coli Biocatalysts ... 
 
www.mdpi.com/1422-0067/13/10/13542/pdf-vor
sites of pRSFDuet-1 (Merck) to obtain pRSFDuet-LSADH. For the co-expression of the qnr and lsadh, E. coli BL21 (DE3) was transformed with pETDuet-QNR and pRSFDuet-LSADH. Unfortunately, we could not construct pETDuet-QNR-LSADH. 2.2.  
 
 
 
Copy number of plasmids with p15A origin of r - Bacteria ... 
 
bionumbers.hms.harvard.edu/bionumber.aspx?id=100260&ver=...
Copy number of plasmids with p15A origin of replication Organism: Bacteria Escherichia coli: Range: 20-30 ... Number of pRSFDuet-1, pRSF plasmids per cell Bacteria Escherichia coli - Range -  
 
 
 
pRSFDuet-1 pRSFDuet1载体质粒图谱、序列、价格 ... 
 
www.biofeng.com/zaiti/dachang/pRSFDuet-1.html
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).  
 
 
 
Reconstitution of Active Mycobacterial Binuclear Iron ... 
 
aem.asm.org/content/79/19/6033.full.pdf
Reconstitution of Active Mycobacterial Binuclear Iron Monooxygenase Complex in Escherichia coli Toshiki Furuya, Mika Hayashi, Kuniki Kino DepartmentofAppliedChemistry,FacultyofScienceandEngineering,WasedaUniversity,Shinjuku-ku,Tokyo,Japan  
 
 
 
Takemura-2015-Biosynthetic routes of hydroxyla_° ¶ָ־ִ¿ג 
 
wenku.baidu.com/view/5e2e5b1fa98271fe910ef999.html
These plasmids were named pET21-MpBHY, pET21-MpCYP97A and pET21MpCYP97C, or pRSFDuet-MpBHY, pRSFDuetMpCYP97A and pRSFDuet-MpCYP97C. We also simultaneously inserted two of the genes MpBHY and MpCYP97C, and MpCYP97A and MpCYP97C into the pRSFDuet ...  
 
 
 
71341 | pRSFDuet™-1 DNA - Novagen 
 
www.merckmillipore.com/INTL/fr/product/pRSFDuet%E2%84%A2...
Description Aperçu pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 
pRSFDuet-1载体_质粒图谱 - 优宝生物 
 
www.youbio.cn/product/vt1238
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).    
 
 
71340 | pCDFDuet™-1 DNA 
 
www.emdmillipore.com/INTL/en/product/pcdfduet-1-dna,EMD...
... β-gal + Fluc; Lane2: pETDuet-1:GST/GUS + GFP; Lane3: pRSFDuet-1:Nus/hlFNγ + S•Tag/T4 PNK; Lane4: pCDFDuet-1:GUS + His•Tag/MBP; Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 ...  
 
 
 
ChemComm Dynamic Article Links - RSC Publishing Home ... 
 
pubs.rsc.org/en/content/articlepdf/2012/cc/c2cc36336d
were inserted into the pRSFDuet-1 vector as a biscistronic construct (garA/garM/pRSFDuet-1) encoding N-terminally His 6-tagged GarA and wild type GarM. Two copies of the garOgenewereinsertedintothepCDFDuet-1vectorencoding proteins lacking an N-terminal hexahistidine tag.  
 
 
 
71341 | pRSFDuet™-1 DNA 
 
www.merckmillipore.com/INTL/en/product/prsfduet-1-dna...
Descripción Visión de conjunto pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).  
 
 
 


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