ecoliwiki.net/colipedia/index.php/pRSFDuet-1 Notes. pRSFDuet-1 is designed for the coexpression of two target proteins. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs.
biochem.web.utah.edu/hill/links/pRSFDuet-1.pdf pRSFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multi-ple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).
www.emdmillipore.com/INTL/en/product/prsfduet-1-dna,EMD... Description Overview pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).
www.sciencedirect.com/science/article/pii/S0960852412011571 They were inserted into the corresponding restriction enzyme sites of plasmid pRSFDuet to give recombinant expression plasmid pRSFDuet-Calb and pRSFDuet-Tll, respectively.
www.nature.com/nmeth/journal/v3/n1/fig_tab/nmeth0106-55... pRSFDuet-1: pCOLADuet-1: pMAL (all) tac: Ampicillin or carbenicillin: 50 g/ml: ColE1 (pBR322) pACYCDuet-1  pCDFDuet-1: pRSFDuet-1: pCOLADuet-1: a Reduce the concentration of antibiotic used by half if transforming more than one plasmid. Next : Close window
www.snapgene.com/resources/plasmid__files/pet_and_duet... pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources".
www.sciencedirect.com/science/article/pii/S0168165613003362 Hence, it is supposed that these should be caused by that the pRSFDuet-1, which had high copy number, resulted in the negative effect on cell physiology and an increase in the metabolic burden of host cells.
www.nature.com/nchembio/journal/v9/n3/full/nchembio.1162... The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry. Weixin Tang 1, 2, Wilfred A van der Donk 1, 2, Affiliations; Contributions; ... Each precursor peptide was expressed as an N-terminal His 6-tagged fusion peptide together with untagged CylM from a pRSFDuet-1 ...
www.biocompare.com/Product-Reviews/...with-pETDuet-1-Duet...... Two kanamycin-resistant Duet vectors are available: pRSFDuet-1 carries the RSF1030 replicon and pCOLADuet-1 carries the ColA replicon. Each vector carries two expression units each controlled by a T7 lac promoter for high-level protein expression.
www.ebiotrade.com/buyf/productsf/Novagen/71341-3.htm pRSFDuet-1 DNA: Cat. No. 71341-3: Product Description: pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding ...
www.ncbi.nlm.nih.gov/pmc/articles/PMC3578037 The enterococcal cytolysin is a two-component lantibiotic of unknown structure with hemolytic activity that is important for virulence. ... Co-expression vector pRSFDuet-1 was obtained from Novagen. Construction of pRSFDuet-1 derivatives for co-expression of CylM and CylL L /CylL S.
www.rsc.org/suppdata/cc/c2/c2cc36336d/c2cc36336d.pdf 3 Overexpression and purification of His6-tagged GarM-modified GarA and GarA-V15L/I16V/19insK E. coli BL21 (DE3) cells transformed with a pRSFDuet-1 vector carrying the genes encoding GarM
faculty.baruch.cuny.edu/dgruber/Research_files/gruber... of pRSFDuet-1, two, and three copies of the lacIq gene were in-serted into the vector (R6 and R7, respectively). R0 serves as a neg-ative control since it lacks a functional lacI gene and contains no operators. Monitoring gene expression in E. coli
www.ncbi.nlm.nih.gov/pmc/articles/PMC3497340 Production of (R)-3-Quinuclidinol by E. coli Biocatalysts Possessing NADH-Dependent 3-Quinuclidinone Reductase (QNR or bacC) from Microbacterium luteolum and Leifsonia Alcohol Dehydrogenase (LSADH)
www.scripps.edu/shen/pdfs/176.pdf Structure of the Bifunctional Acyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity ... The pRSFDuet-1 plasmid and Escherichia coli NovaBlue and BL21(DE3) cells were from Novagen (Madison, WI). Primer synthesis and DNA Figure 1.
www.researchgate.net/publictopics.PublicPostFileLoader... cloned into pRSFDuet-1 were substantially reduced in coexpression experiments with pETDuet-1 and pACYCDuet-1 in the same cell. A significantly different pattern of expression was obtained when the lower copy number pCOLADuet™-1 was substituted for pRSFDuet-1 in an otherwise
guide5.net/j/july---university-of-kansas-medical-center... JARID1A-PHD3 was fused to the C terminus of a His(63)-SUMO tag in a modified pRSFDuet-1 vector (Novagen), with a ubiquitin-like-protease (ULP)
ecoliwiki.net/colipedia/index.php/pACYCDuet-1 This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.. History .
2012.igem.org/Team:SJTU-BioX-Shanghai/Project/project1.1 Reconstructed Vectors. Vectors used in the project for membrane protein expression are modified versions of pRSFDuet-1, pETDuet-1,pACYCDuet-1(NOVAGEN), which are originally regulated by T7 promoter.
www.biotechnologyforbiofuels.com/content/8/1/34 The plasmids pACYCDuet-1, pRSFDuet-1, and pET-22(b) were obtained from Novagen (Darmstadt, Germany). Recombinant plasmid pET-28(b)-oleT JE for expression of the P450 fatty acid decarboxylase OleT JE was constructed in our laboratory previously . E. coli ...
www.jbc.org/content/286/1/867.full.pdf pRSFDuet-1-Fra1-His 6 /Fra2 1–35 plasmids were used for ex-pression and purification of apo-Fra2. As noted previously, Fra1-His 6 is not expressed at detectable levels from pRSF-Duet-1-Fra1-His 6 /Fra2 plasmids (9). Nevertheless, these co-
abbs.oxfordjournals.org/content/suppl/2012/05/26/gms047... pRSFDuet-1 T7lac promoter followed by two multiple cloning sites (MCS), Novagen. Carries the RSF1030 replicon, lacI and kanR . pRDUPBC pRSFDuet-1 with accPB cloned in EcoRV-XhoI sites this study. and accC cloned in BamHI-HindIII ...
www.jci.org/articles/view/75572/figure/1 STXBP5 is present in human platelets. (A) The indicated complexes were produced in E. coli using the pRSFDuet plasmid with the S-tag peptide fused to the C terminus of the syntaxin.
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www.pnas.org/content/suppl/2012/03/13/1116815109.DC... pRSFDuet-1 (GeoAI/GeoB) and pACYCDuet-1 (GeoC) were picked and grown in LB media overnight. The culture was used to inoculate a 6-L culture in Terrific Broth media containing 50 mg∕L kanamycin and 34 mg∕L chloramphenicol.
www.jbc.org/content/287/43/36544.full.pdf pRSFDuet-1/hydG Expression of untagged HydG protein pRSFDuet-1/hydG-6His Expression of a HydG protein with a N-terminal 6His tag pETDuet-1/hydA1-StrepII Expression of a HydA1 protein with a C-terminal StrepII tag
homepages.rpi.edu/~koffam/papers/2012_Malla_Koffas.pdf Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable Flavonoid, in Escherichia coli ... pRSFDuet-1 Double T7 promoters; RSF ori Kmr Novagen pET24ma( r) Expression vector with T7 promoter; p15A ori Km Pasteur Institute, France
www.mdpi.com/1422-0067/13/10/13542/pdf-vor sites of pRSFDuet-1 (Merck) to obtain pRSFDuet-LSADH. For the co-expression of the qnr and lsadh, E. coli BL21 (DE3) was transformed with pETDuet-QNR and pRSFDuet-LSADH. Unfortunately, we could not construct pETDuet-QNR-LSADH. 2.2.
bionumbers.hms.harvard.edu/bionumber.aspx?id=100260&ver=... Copy number of plasmids with p15A origin of replication Organism: Bacteria Escherichia coli: Range: 20-30 ... Number of pRSFDuet-1, pRSF plasmids per cell Bacteria Escherichia coli - Range -
www.biofeng.com/zaiti/dachang/pRSFDuet-1.html pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).
aem.asm.org/content/79/19/6033.full.pdf Reconstitution of Active Mycobacterial Binuclear Iron Monooxygenase Complex in Escherichia coli Toshiki Furuya, Mika Hayashi, Kuniki Kino DepartmentofAppliedChemistry,FacultyofScienceandEngineering,WasedaUniversity,Shinjuku-ku,Tokyo,Japan
wenku.baidu.com/view/5e2e5b1fa98271fe910ef999.html These plasmids were named pET21-MpBHY, pET21-MpCYP97A and pET21MpCYP97C, or pRSFDuet-MpBHY, pRSFDuetMpCYP97A and pRSFDuet-MpCYP97C. We also simultaneously inserted two of the genes MpBHY and MpCYP97C, and MpCYP97A and MpCYP97C into the pRSFDuet ...
www.merckmillipore.com/INTL/fr/product/pRSFDuet%E2%84%A2... Description Aperçu pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).
www.youbio.cn/product/vt1238 pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs).
pubs.rsc.org/en/content/articlepdf/2012/cc/c2cc36336d were inserted into the pRSFDuet-1 vector as a biscistronic construct (garA/garM/pRSFDuet-1) encoding N-terminally His 6-tagged GarA and wild type GarM. Two copies of the garOgenewereinsertedintothepCDFDuet-1vectorencoding proteins lacking an N-terminal hexahistidine tag.
www.merckmillipore.com/INTL/en/product/prsfduet-1-dna... Descripción Visión de conjunto pRSFDuet™-1 is designed for the coexpression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).