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71363 | pRSF-1b DNA 
 
www.merckmillipore.com/INTL/en/product/prsf-1b-dna,EMD...
Description Overview The pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag ® coding sequence that results in native protein after purification and cleavage.  
 
 
 
pRSF-1b Sequence and Map - SnapGene | Software for everyday ... 
 
www.snapgene.com/.../pet_and_duet_vectors_(novagen)/pRSF-1b
pRSF-1b Bacterial vector with an RSF 1030 origin for the regulated expression of N-terminally 6xHis-tagged proteins.  
 
 
 
pRSF and pCDF Vectors - 生物通 ... 
 
www.ebiotrade.com/buyf/productsf/Novagen/tb401-000.pdf
pRSF and pCDF Vectors pRSF-1b and pCDF-1b Each plasmid carries a T7lac promoter, ribosome binding site, multiple cloning site (MCS) and T7  
 
 
 
Novagen - Helmholtz Zentrum München 
 
www.helmholtz-muenchen.de/.../pRSF_vectors/pRSF-1b_map.pdf
pRSF-1b carries a T7 promoter and lac operator to control transcription, a replication origin derived from RSF1030, and kanamycin antibiotic resistance (KnR). It also encodes an N-terminal His•Tag ...  
 
 
 
In-cell NMR for protein-protein interactions (STINT-NMR ... 
 
www.nature.com/nprot/journal/v1/n1/full/nprot.2006.23.html
Top Materials Reagents. Plasmid pRSF.1b (Novagen), containing the cDNA for the interactor protein of interest; Plasmid pBADHisA (Invitrogen), containing the cDNA for the target protein of interest  
 
 
 
Effects of cytoplasmic and periplasmic chaperones on ... 
 
www.sciencedirect.com/science/article/pii/S1389172310004196
The effects of cytoplasmic and periplasmic chaperones on the secretory production of an anti-bovine ribonuclease A single-chain variable ... BugBuster protein extraction reagent and the plasmids pCDF-1b and pRSF-1b were purchased from Novagen (Madison, WI, USA). The chaperone plasmids pGro7 ...  
 
 
 
pRSF-2 Ek/LIC - EcoliWiki 
 
ecoliwiki.net/colipedia/index.php/pRSF-2_Ek/LIC
Notes. The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.  
 
 
 
71375 | pRSF-1 Expression System 
 
www.merckmillipore.com/INTL/en/product/prsf-1-expression...
Description Overview This product has been discontinued. The pRSF-1 Expression System contains 10 µg of pRSF-1b DNA. pRSF-1b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ sequence.  
 
 
 
CRISPR Interference Directs Strand Specific Spacer Acquisition 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3338789
Spacer Integration Results in Plasmid Curing and Plasmid Interference. Upon prolonged cultivation (~1–2 weeks) at 37°C under non-selective conditions E. coli Δhns is cured from the 3.7 kb high copy number plasmid pRSF-1b.  
 
 
 
METHODS - Nature Publishing Group : science journals, jobs ... 
 
www.nature.com/nmeth/journal/v3/n2/extref/nmeth851-S8.doc
using the oligonucleotides 5'-GTAGCAGTCGACCTACAGGAGAGG-3' and 5'-TTTTTTCCATGGCTCTGTTCACTGC-3' and ligated into pRSF-1b (Novagen) using the SalI and NcoI linker sites ... pRSF-AUIM(SA16) or pRSF-STAM2. This strain contains the pRARE plasmid, which over-expresses tRNAs for Arg, Leu, and Pro codons ...  
 
 
 
PLOS ONE: CRISPR Interference Directs Strand Specific Spacer ... 
 
www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal...
Results and Discussion Spacer Integration Results in Plasmid Curing and Plasmid Interference. Upon prolonged cultivation (~1–2 weeks) at 37°C under non-selective conditions E. coli Δhns is cured from the 3.7 kb high copy number plasmid pRSF-1b.  
 
 
 
pRSF-1 Expression System - 生物通 ... 
 
www.ebiotrade.com/buyf/productsf/Novagen/71375-3.htm
Product Description: The pRSF-1 Expression System contains 10 µg of pRSF-1b DNA. pRSF-1b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ sequence.  
 
 
 
Host cell and expression engineering for development of an E ... 
 
www.ncbi.nlm.nih.gov/pmc/articles/PMC3278346
We chose pRSF-1b, an expression system for protein expression of up to 50% of the total E. coli protein due to a copy number of > 100 and the strong T7lac promoter. The constructed pRSF-1b_FDH was transformed into BL21 (DE3), ...  
 
 
 
Bioscience Price List 2014-15 – Volume I 
 
vakilspremedia.com/merck_bioscience/pricelist-2014-15...
pRSF-1b, are also available with this minimal sequence feature. These vectors differ from pET-45b(+) in that they have different. replicons and drug resistance genes. These two features make the. pCDF-1b and pRSF-1b vectors suitable for coexpression with each.  
 
 
 
RESEARCH Open Access Host cell and expression engineering for ... 
 
link.springer.com/content/pdf/10.1186%2F1475-2859-11-7
chose pRSF-1b, an expression system for protein expres-sion of up to 50% of the total E. coli protein due to a copy number of > 100 and the strong T7 lac promoter. The con-structed pRSF-1b_FDH was transformed into BL21 (DE3),  
 
 
 
An engineered E.coli strain for the production of ... 
 
www.sciencedirect.com/science/article/pii/S1096717612000663
The glycolipid synthase MG517 from Mycoplasma genitalium catalyzes the glucosyl transfer from UDPGlc to diacylglycerol producing glycoglycerolipids (GGL) ( Andr  
 
 
 
Protein Expression and Purification Facility (PEPF ... 
 
www.helmholtz-muenchen.de/en/pepf/materials/vector...
pRSF-1b: map: T7/lac: Kan: N-His: TEV: BL21 (DE3) RSF1030: Novagen: expression vector: pRSF-11: map: T7/lac: Kan: N-His: TEV: BL21 (DE3) RSF1030: EMBL-AG: expression vector: pRSF-13: map: T7/lac: Kan: C-His: TEV: BL21 (DE3) RSF1030: EMBL-AG: expression vector: pRSFDuet-1: map: T7/lac: Kan: N-His ...  
 
 
 
Supporting Information 
 
www.pnas.org/content/suppl/2011/02/15/1014934108.DC...
K187N) were introduced into either the pRSF-1b or the pET-51b(+) vector (Novagen). pRSF-1b encodes an N-terminal His 6-tag, an RSF origin of replication and kanamycin resistance, whereas pET-51b(+) encodes an N-terminal Strep tag, an F1  
 
 
 
Changing the substrate specificity of P450cam towards ... 
 
peds.oxfordjournals.org/content/24/5/439.full.pdf
Changing the substrate specificity of P450cam towards diphenylmethane by semi-rational enzyme engineering Gregor Hoffmann1, Kathrin Bo¨nsch1, Thomas Greiner-Sto¨ffele1,2 and Meike Ballschmiter1,3  
 
 
 
pET & Duet Vectors ( Novagen ) - SnapGene | Software for ... 
 
www.snapgene.com/resources/plasmid__files/pet_and_duet...
pRSF-1b; pRSF-2 Ek/LIC; pRSF-2 Ek/LIC (linearized) pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material.  
 
 
 
Changing the substrate specificity of P450cam towards ... 
 
peds.oxfordjournals.org/content/24/5/439.full
Changing the substrate specificity of P450cam towards ...  
 
 
 
Flor De La Cruz, Mentor: Amedeo Veter PI: Bridget Wagner ... 
 
www.broadinstitute.org/files/shared/diversity/summer...
RNA extraction from mouse islets Reverse Transcription and PCR Clone GCK cDNA into pRSF-1b Express recombinant GCK protein  
 
 
 
71364 | pRSF-2 Ek/LIC Vector Kit 
 
www.emdmillipore.com/.../prsf-2-ek-lic-vector-kit,EMD_BIO......
pRSF-2 Ek/LIC Vector Kit. Switzerland / English. Quick Purchase; My Favorites; Contact Us Search. Login. Register. 0. Compare. 0 Cart. Home; Products Analytics and Sample Preparation Chromatography for Analysis Analytical Sample Preparation ...  
 
 
 
Host cell and expression engineering for development of an E ... 
 
link.springer.com/article/10.1186/1475-2859-11-7/...
Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH  
 
 
 
Production of the Potent Antibacterial Polyketide ... 
 
aem.asm.org/content/71/5/2539.full.pdf
from pRSF-1b (Novagen) to accept XbaI/EcoRI fragments as follows. (i) The XbaI site at nucleotide 2254 was eliminated by cleavage, filling-in, and blunt end ligation; (ii) the MluI/BlpI fragment of the polylinker was replaced with that  
 
 
 
71363 | pRSF-1b DNA 
 
www.emdmillipore.com/INTL/en/product/prsf-1b-dna,EMD_BIO...
Descripción Visión de conjunto The pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag ® coding sequence that results in native protein after purification and cleavage.  
 
 
 
Functional replacement of the endogenous tyrosyl-tRNA ... 
 
nar.oxfordjournals.org/content/38/11/3682.full-text...
Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion  
 
 
 
YidC--a molecular chaperone for LacY protein folding via the ... 
 
www.jbc.org/content/early/2013/08/08/jbc.M113.491613...
Company. pRSF-1b vector was from Novagen Company. The pT7-5 expression vector containing the lacY cassette encoding Cys-less LacY (with a His tag at the C-terminus) under the control of T7/Lac promoter was from the Kaback collection. The ...  
 
 
 
CRISPR Interference Directs Strand Specific Spacer Acquisition 
 
edepot.wur.nl/210355
CRISPR Interference Directs Strand Specific Spacer Acquisition ... pRSF-1b in the corresponding regions excluded the possibility that the plasmid contained mutations at these positions, confirm-ing that these PAMs were indeed non-consensus PAM sequences.    
 
 
BRCA1-Associated Protein 1 Interferes with BRCA1/BARD1 RING ... 
 
cancerres.aacrjournals.org/content/69/1/111.long
Cells were then transformed with pRSF-1b-His-BARD1 1-320 and selected with ampicillin and kanamycin. His-FLAG-BRCA1 1-304 was first expressed by IPTG induction at 15°C for 24 h followed by expression of His-BARD1 1-320 at 25°C for 16 h.  
 
 
 
Engineering an Acinetobacterregulon for biosensing and high ... 
 
nar.oxfordjournals.org/content/early/2014/05/26/nar.gku...
Engineering an Acinetobacterregulon for biosensing and high-throughput enzyme screening in E. colivia flow cytometry ... wassubclonedintopRSF-1bvector(Novagen)attheSacI ... pRSF.asbf,wastransformedinE.colicellsthatalreadycon-  
 
 
 
pHYRSF1-02 - Addgene - Homepage 
 
www.addgene.org/24765
pRSF-1b (Invitrogen) (Search Vector Database) Backbone size ... pHYRSF1-02 was a gift from Hideo Iwai (Addgene plasmid # 24765 ...    
 
 
www.albany.edu 
 
www.albany.edu/honorscollege/files/Kartis_Honors_Thesis...
The pRSF.1b expression vector from Novagen was chosen for a number of reasons. It is a high copy number plasmid, confers Kanamycin resistance, contains an RSF origin and has an inducible T7 promoter. The plasmid map of the pRSF.1b vector is shown in Figure 9.  
 
 
 
HexamericCalgranulinC(S100A12)BindstotheReceptorfor ... 
 
www.jbc.org/content/282/6/4218.full.pdf
SalI sites of expression vector pRSF-1b (Novagen). Both plas-mids confer kanamycin resistance. The resulting plasmids pET28sRAGE, pET28V, pET28C1, pET28C2, pET28VC1, and pET28C1C2expresscleavable,C-terminalHis-taggedproteins. pRSF-HMGB1 plasmid expresses full-length human HMGB1  
 
 
 
In vivo characterization of the dTDP-D-desosamine pathway of ... 
 
mic.sgmjournals.org/content/152/3/667.full.pdf
was cloned as an XbaI–EcoRI fragment into the pRSF-1b-derived pKOS431-39-1 (Peiru´ et al., 2005), compatible with both pET-and pCDF-derived vectors. Then two expression vectors were constructed as described by Peiru´ et al. (2005) containing the sets  
 
 
 
Supporting Information - PNAS 
 
www.pnas.org/content/suppl/2011/05/31/1104144108.DC...
pWUR408 casA in pRSF-1b, no tags Brouns et al. (1) pWUR514 casB with Strep-tag II (N-term)-casC-casD-CasE in pET52b Jore et al. (2) pWUR610 lambda fragment 17918–18250, in pUC19 BamHI/HindIII BG3218 + BG3219 this study  
 
 
 
Production of the Potent Antibacterial Polyketide ... 
 
aem.asm.org/content/71/5/2539.full
pKOS431-54-2 was derived from pCDF-1b (Novagen) to modify the antibiotic resistance and to accept XbaI/EcoRI fragments as follows. (i) The XbaI site at ... a derivative of pRSF-1b (Novagene), to give pKOS452-53e. ...    
 
 
Supporting Online Material for - Davidson College 
 
gcat.davidson.edu/sybr-u/Brouns_SOM.pdf
pRSF-1b T7 RNA polymerase based expression vector, Kan R Novagen pCDF-1b T7 RNA polymerase based expression vector, Str R Novagen pACYCduet-1 T7 RNA polymerase based expression vector, Cam R Novagen pZErO-1.1 Cloning plasmid, Zeo R Invitrogen  
 
 
 
Patent US7582475 - Vectors and methods for high throughput co ... 
 
www.google.com/patents/US7582475
The PshAI restriction site present in both the pCDF-1b and pRSF-1b vectors was used for the insertion of the Gateway® cassette. ... "Novagen: TB392: pCDF-1b Vector Map," 3 pages. 3 "Novagen: TB401:pRSF-1 And pCDF-1 Vectors for Coexpression.    
 
 
PLOS ONE: Fate of Pup inside the Mycobacterium Proteasome ... 
 
www.plosone.org/article/info:doi/10.1371/journal.pone...
The gene was ligated into pRSF-1b [Novagen] using the KpnI and HindIII linker sites. The resulting plasmid, pRSF-Msm Mpa, expresses N-terminal His-tagged Msm Mpa from P T7 /lacOp, which is induced by IPTG.  
 
 
 
Helicase dissociation and annealing of RNA-DNA hybrids by ... 
 
www.academia.edu/10000657/Helicase_dissociation_and...
Cloning for in vivo phage protection assay Cloning of E. coli MG1655 cas3 into pRSF-1b required prior manipulation to remove an NcoI site from within the coding sequence of cas3. Deletion of the ...  
 
 
 
Immunization against a merozoite sheddase promotes multiple ... 
 
www.malariajournal.com/content/pdf/1475-2875-13-313.pdf
and BamHI restriction sites into a modified pRSF-1b vector (EMD Millipore, Billerica, MA) for expression as a maltose binding protein (MBP)-fusion protein with a C-terminal 6×His tag for purification and detection purposes as previously described [17].  
 
 
 
The Binding of Antibiotics in OmpF Porin | Benoit Roux ... 
 
www.academia.edu/5113347/The_Binding_of_Antibiotics_in...
Disk Diffusion and Agar Dilution Antibiotic Susceptibility Assays E. coli BL21(DE3)Omp5 bacteria transformed with pRSF-1b plasmid encoding the WT or mutant OmpF protein sequence were incubated in LB broth supple- mented with kanamycin (30 mg/ml), at 37 C, 250 rpm.  
 
 
 
Patent US20110052758 - Heat-stable asparaginase for reducing ... 
 
www.google.com/patents/US20110052758
The invention relates to the use of an amidohydrolase for preparing foodstuffs or stimulants.  
 
 
 
Addgene: Hideo Iwai Lab Plasmids 
 
www.addgene.org/Hideo_Iwai
Hideo Iwai Lab Plasmids Create a link to this page ... pRSF-1b (Invitrogen) Add to Cart: 31122: pHYRS52: Ulp1 (Saccharomyces cerevisiae) Bacterial Expression: Segmental isotopic labelling of a multidomain protein by protein ligation by ...    
 
 
CRISPR interference directs strand specific spacer ... 
 
www.researchgate.net/publication/224899484_CRISPR...
CRISPR interference directs strand specific spacer acquisition. Daan C Swarts . Cas Mosterd . Mark W J van Passel . Stan J J Brouns . Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen ...  
 
 
 
Functional replacement of the endogenous tyrosyl-tRNA ... 
 
europepmc.org/articles/PMC2887954
Functional replacement of the endogenous tyrosyl-tRNA ...    
 
 
Sec1/Munc18 protein Vps33 binds to SNARE domains and the ... 
 
www.molbiolcell.org/content/23/23/4611.full
Vti1 was cloned into the pRSF-1b vector with a N-terminal His 7-MBP (maltose-binding protein) tag separated with a short linker and TEV recognition site. ... (2010) The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.  
 
 
 
Marin Dwarf Flax - Presidio of San Francisco (U.S. National ... 
 
www.nps.gov/prsf/naturescience/marin-dwarf-flax.htm
Status: Federal: Proposed Threatened State: Threatened California Native Plant Society: List 1B, R-E-D Code 3-3-3 (see below) 3. Occurrence limited to one or a few highly restricted populations or present in such small numbers that it is seldom reported.  
 
 
 
Escherichia coli expression vectors | my-whiteboard 
 
www.my-whiteboard.com/escherichia-coli-expression-vectors
pRSF-1b: map: T7/lac: Kan: N-His: TEV: BL21 (DE3) RSF1030: Novagen: expression vector: pRSF-11: map: T7/lac: Kan: N-His: TEV: BL21 (DE3) RSF1030: EMBL-AG: expression vector: pRSF-13: map: T7/lac: Kan: C-His: TEV: BL21 (DE3) RSF1030: EMBL-AG: expression vector: pRSFDuet-1: map: T7/lac: Kan: N-His ...  
 
 
 


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