www.merckmillipore.com/INTL/en/product/prsf-1b-dna,EMD... Description Overview The pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag ® coding sequence that results in native protein after purification and cleavage.
www.helmholtz-muenchen.de/.../pRSF_vectors/pRSF-1b_map.pdf pRSF-1b carries a T7 promoter and lac operator to control transcription, a replication origin derived from RSF1030, and kanamycin antibiotic resistance (KnR). It also encodes an N-terminal His•Tag ...
www.nature.com/nprot/journal/v1/n1/full/nprot.2006.23.html Top Materials Reagents. Plasmid pRSF.1b (Novagen), containing the cDNA for the interactor protein of interest; Plasmid pBADHisA (Invitrogen), containing the cDNA for the target protein of interest
www.sciencedirect.com/science/article/pii/S1389172310004196 The effects of cytoplasmic and periplasmic chaperones on the secretory production of an anti-bovine ribonuclease A single-chain variable ... BugBuster protein extraction reagent and the plasmids pCDF-1b and pRSF-1b were purchased from Novagen (Madison, WI, USA). The chaperone plasmids pGro7 ...
ecoliwiki.net/colipedia/index.php/pRSF-2_Ek/LIC Notes. The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.
www.merckmillipore.com/INTL/en/product/prsf-1-expression... Description Overview This product has been discontinued. The pRSF-1 Expression System contains 10 µg of pRSF-1b DNA. pRSF-1b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ sequence.
www.ncbi.nlm.nih.gov/pmc/articles/PMC3338789 Spacer Integration Results in Plasmid Curing and Plasmid Interference. Upon prolonged cultivation (~1–2 weeks) at 37°C under non-selective conditions E. coli Δhns is cured from the 3.7 kb high copy number plasmid pRSF-1b.
www.nature.com/nmeth/journal/v3/n2/extref/nmeth851-S8.doc using the oligonucleotides 5'-GTAGCAGTCGACCTACAGGAGAGG-3' and 5'-TTTTTTCCATGGCTCTGTTCACTGC-3' and ligated into pRSF-1b (Novagen) using the SalI and NcoI linker sites ... pRSF-AUIM(SA16) or pRSF-STAM2. This strain contains the pRARE plasmid, which over-expresses tRNAs for Arg, Leu, and Pro codons ...
www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal... Results and Discussion Spacer Integration Results in Plasmid Curing and Plasmid Interference. Upon prolonged cultivation (~1–2 weeks) at 37°C under non-selective conditions E. coli Δhns is cured from the 3.7 kb high copy number plasmid pRSF-1b.
www.ebiotrade.com/buyf/productsf/Novagen/71375-3.htm Product Description: The pRSF-1 Expression System contains 10 µg of pRSF-1b DNA. pRSF-1b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ sequence.
www.ncbi.nlm.nih.gov/pmc/articles/PMC3278346 We chose pRSF-1b, an expression system for protein expression of up to 50% of the total E. coli protein due to a copy number of > 100 and the strong T7lac promoter. The constructed pRSF-1b_FDH was transformed into BL21 (DE3), ...
vakilspremedia.com/merck_bioscience/pricelist-2014-15... pRSF-1b, are also available with this minimal sequence feature. These vectors differ from pET-45b(+) in that they have different. replicons and drug resistance genes. These two features make the. pCDF-1b and pRSF-1b vectors suitable for coexpression with each.
link.springer.com/content/pdf/10.1186%2F1475-2859-11-7 chose pRSF-1b, an expression system for protein expres-sion of up to 50% of the total E. coli protein due to a copy number of > 100 and the strong T7 lac promoter. The con-structed pRSF-1b_FDH was transformed into BL21 (DE3),
www.sciencedirect.com/science/article/pii/S1096717612000663 The glycolipid synthase MG517 from Mycoplasma genitalium catalyzes the glucosyl transfer from UDPGlc to diacylglycerol producing glycoglycerolipids (GGL) ( Andr
www.pnas.org/content/suppl/2011/02/15/1014934108.DC... K187N) were introduced into either the pRSF-1b or the pET-51b(+) vector (Novagen). pRSF-1b encodes an N-terminal His 6-tag, an RSF origin of replication and kanamycin resistance, whereas pET-51b(+) encodes an N-terminal Strep tag, an F1
peds.oxfordjournals.org/content/24/5/439.full.pdf Changing the substrate speciﬁcity of P450cam towards diphenylmethane by semi-rational enzyme engineering Gregor Hoffmann1, Kathrin Bo¨nsch1, Thomas Greiner-Sto¨ffele1,2 and Meike Ballschmiter1,3
www.snapgene.com/resources/plasmid__files/pet_and_duet... pRSF-1b; pRSF-2 Ek/LIC; pRSF-2 Ek/LIC (linearized) pRSFDuet-1. The map, notes, and annotations in the zip file on this page are copyrighted material.
link.springer.com/article/10.1186/1475-2859-11-7/... Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH
aem.asm.org/content/71/5/2539.full.pdf from pRSF-1b (Novagen) to accept XbaI/EcoRI fragments as follows. (i) The XbaI site at nucleotide 2254 was eliminated by cleavage, ﬁlling-in, and blunt end ligation; (ii) the MluI/BlpI fragment of the polylinker was replaced with that
www.emdmillipore.com/INTL/en/product/prsf-1b-dna,EMD_BIO... Descripción Visión de conjunto The pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag ® coding sequence that results in native protein after purification and cleavage.
nar.oxfordjournals.org/content/38/11/3682.full-text... Functional replacement of the endogenous tyrosyl-tRNA synthetase–tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion
www.jbc.org/content/early/2013/08/08/jbc.M113.491613... Company. pRSF-1b vector was from Novagen Company. The pT7-5 expression vector containing the lacY cassette encoding Cys-less LacY (with a His tag at the C-terminus) under the control of T7/Lac promoter was from the Kaback collection. The ...
edepot.wur.nl/210355 CRISPR Interference Directs Strand Specific Spacer Acquisition ... pRSF-1b in the corresponding regions excluded the possibility that the plasmid contained mutations at these positions, confirm-ing that these PAMs were indeed non-consensus PAM sequences.
cancerres.aacrjournals.org/content/69/1/111.long Cells were then transformed with pRSF-1b-His-BARD1 1-320 and selected with ampicillin and kanamycin. His-FLAG-BRCA1 1-304 was first expressed by IPTG induction at 15°C for 24 h followed by expression of His-BARD1 1-320 at 25°C for 16 h.
nar.oxfordjournals.org/content/early/2014/05/26/nar.gku... Engineering an Acinetobacterregulon for biosensing and high-throughput enzyme screening in E. colivia ﬂow cytometry ... wassubclonedintopRSF-1bvector(Novagen)attheSacI ... pRSF.asbf,wastransformedinE.colicellsthatalreadycon-
www.albany.edu/honorscollege/files/Kartis_Honors_Thesis... The pRSF.1b expression vector from Novagen was chosen for a number of reasons. It is a high copy number plasmid, confers Kanamycin resistance, contains an RSF origin and has an inducible T7 promoter. The plasmid map of the pRSF.1b vector is shown in Figure 9.
mic.sgmjournals.org/content/152/3/667.full.pdf was cloned as an XbaI–EcoRI fragment into the pRSF-1b-derived pKOS431-39-1 (Peiru´ et al., 2005), compatible with both pET-and pCDF-derived vectors. Then two expression vectors were constructed as described by Peiru´ et al. (2005) containing the sets
www.pnas.org/content/suppl/2011/05/31/1104144108.DC... pWUR408 casA in pRSF-1b, no tags Brouns et al. (1) pWUR514 casB with Strep-tag II (N-term)-casC-casD-CasE in pET52b Jore et al. (2) pWUR610 lambda fragment 17918–18250, in pUC19 BamHI/HindIII BG3218 + BG3219 this study
aem.asm.org/content/71/5/2539.full pKOS431-54-2 was derived from pCDF-1b (Novagen) to modify the antibiotic resistance and to accept XbaI/EcoRI fragments as follows. (i) The XbaI site at ... a derivative of pRSF-1b (Novagene), to give pKOS452-53e. ...
gcat.davidson.edu/sybr-u/Brouns_SOM.pdf pRSF-1b T7 RNA polymerase based expression vector, Kan R Novagen pCDF-1b T7 RNA polymerase based expression vector, Str R Novagen pACYCduet-1 T7 RNA polymerase based expression vector, Cam R Novagen pZErO-1.1 Cloning plasmid, Zeo R Invitrogen
www.google.com/patents/US7582475 The PshAI restriction site present in both the pCDF-1b and pRSF-1b vectors was used for the insertion of the Gateway® cassette. ... "Novagen: TB392: pCDF-1b Vector Map," 3 pages. 3 "Novagen: TB401:pRSF-1 And pCDF-1 Vectors for Coexpression.
www.plosone.org/article/info:doi/10.1371/journal.pone... The gene was ligated into pRSF-1b [Novagen] using the KpnI and HindIII linker sites. The resulting plasmid, pRSF-Msm Mpa, expresses N-terminal His-tagged Msm Mpa from P T7 /lacOp, which is induced by IPTG.
www.academia.edu/10000657/Helicase_dissociation_and... Cloning for in vivo phage protection assay Cloning of E. coli MG1655 cas3 into pRSF-1b required prior manipulation to remove an NcoI site from within the coding sequence of cas3. Deletion of the ...
www.malariajournal.com/content/pdf/1475-2875-13-313.pdf and BamHI restriction sites into a modified pRSF-1b vector (EMD Millipore, Billerica, MA) for expression as a maltose binding protein (MBP)-fusion protein with a C-terminal 6×His tag for purification and detection purposes as previously described .
www.academia.edu/5113347/The_Binding_of_Antibiotics_in... Disk Diffusion and Agar Dilution Antibiotic Susceptibility Assays E. coli BL21(DE3)Omp5 bacteria transformed with pRSF-1b plasmid encoding the WT or mutant OmpF protein sequence were incubated in LB broth supple- mented with kanamycin (30 mg/ml), at 37 C, 250 rpm.
www.addgene.org/Hideo_Iwai Hideo Iwai Lab Plasmids Create a link to this page ... pRSF-1b (Invitrogen) Add to Cart: 31122: pHYRS52: Ulp1 (Saccharomyces cerevisiae) Bacterial Expression: Segmental isotopic labelling of a multidomain protein by protein ligation by ...
www.researchgate.net/publication/224899484_CRISPR... CRISPR interference directs strand specific spacer acquisition. Daan C Swarts . Cas Mosterd . Mark W J van Passel . Stan J J Brouns . Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen ...
www.molbiolcell.org/content/23/23/4611.full Vti1 was cloned into the pRSF-1b vector with a N-terminal His 7-MBP (maltose-binding protein) tag separated with a short linker and TEV recognition site. ... (2010) The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
www.nps.gov/prsf/naturescience/marin-dwarf-flax.htm Status: Federal: Proposed Threatened State: Threatened California Native Plant Society: List 1B, R-E-D Code 3-3-3 (see below) 3. Occurrence limited to one or a few highly restricted populations or present in such small numbers that it is seldom reported.